慢性饮水染镉对大鼠血液及睾丸中某些酶活性的影响

发布时间：2018-05-02 05:32

本文选题：镉(Cd) + 血液　；参考：《黑龙江畜牧兽医》2017年07期

【摘要】：为了探讨慢性饮水染镉(Cd)对大鼠血液、睾丸中某些酶活性的影响,试验将30只近交系SD大鼠(雄性,170 g左右)随机分成5组,即对照组、试验1组、试验2组、试验3组、试验4组,分别饮用Cd(Cd Cl2)质量浓度为0,25,50,100,200 mg/L的饮水。56 d后宰杀大鼠,测定血液中和睾丸中镉的蓄积量及碱性磷酸酶(ALP)、乳酸脱氢酶同工酶-X(LDH-X)、酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)、葡萄糖-6-磷酸脱氢酶(G-6-PD)、山梨醇脱氢酶(SDH)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、γ-L-谷氨酰转肽酶(GGT)活性。结果表明:与对照组相比,试验1组大鼠血浆LDH活性降低;血浆AST和ALT活性的剂量效应不规律,试验2组有降低现象,而且均只有试验4组显著升高(P0.05)。与对照组相比,试验2组睾丸中ALP活性显著降低(P0.05);试验3组中,除LDH-X和ACP外,其他酶活性均显著降低(P0.05);试验4组各酶活性均显著降低(P0.05)。慢性饮水染镉,睾丸中某些酶活性的改变较血液中敏感,睾丸中LDH和ALP可以作为反映慢性饮水染镉雄性生殖毒性较敏感的生化指标。
[Abstract]:In order to investigate the effect of chronic drinking water on the activities of some enzymes in blood and testis of rats, 30 inbred SD rats (about 170g male) were randomly divided into five groups: control group, experimental group 1, experimental group 2 and experimental group 3. In the four groups, the rats were slaughtered after drinking Cd(Cd Cl _ 2 (0.25g / 100,200 mg/L) for 56 d, respectively. The contents of cadmium in blood and testis and alkaline phosphatase (ALPN), lactate dehydrogenase isoenzyme (lactic dehydrogenase isoenzyme) -XnLDH-XN, acid phosphatase (ACPH), lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G-6-PDN), sorbitol dehydrogenase (SDH), aspartate aminotransferase (AST) were determined. Alanine aminotransferase (alt), 纬-L-glutamyl transpeptidase (GGT) activity. The results showed that compared with the control group, the plasma LDH activity and the dose-effect of plasma AST and ALT activities in the first group were decreased, but only in the fourth group the plasma AST and ALT activities were significantly increased (P 0.05). Compared with the control group, the activity of ALP in testes of experimental group 2 was significantly lower than that of control group, the activity of other enzymes except LDH-X and ACP was significantly decreased in group 3, and that of each enzyme in group 4 was significantly lower than that of control group. The changes of some enzyme activities in testis were more sensitive than those in blood in chronic drinking water, and LDH and ALP in testis could be regarded as sensitive biochemical indexes to reflect the male reproductive toxicity of cadmium in chronic drinking water.
【作者单位】：扬州大学兽医学院;江苏省动物重要疫病与人兽共患病防控协同创新中心;
【基金】：江苏省普通高校自然科学基金项目(08KJD230002) 江苏高校优势学科建设工程资助项目(PAPD)
【分类号】：R114

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