3种淡水环境中低核酸含量细菌的滤过性

发布时间：2018-05-03 15:23

  本文选题：流式细胞技术(FCM) + 低核酸含量(LNA)细菌　； 参考：《微生物学通报》2016年09期

【摘要】：【目的】增加低核酸含量(LNA)细菌与过滤性细菌之间的认识。【方法】采用流式细胞技术(FCM)、变性梯度凝胶电泳(DGGE)及统计学分析研究3种典型淡水环境中细菌群落与滤过性。【结果】LNA细菌与过滤性细菌在数量上具有很好的相关性(y=0.646x+22.42,R2=0.984,P0.01),细菌的滤过性不仅与细菌大小有关,还与细胞整体形状及其伸缩性有关;细菌群落组织与LNA细菌比重呈负相关性,而与HNA细菌呈正相关性。【结论】0.45μm膜过滤对水样微生物群落中的LNA细菌具有极强的筛选效果;细菌群落组织与其基于流式细胞技术测定的基因含量具有密切联系。
[Abstract]:[objective] to increase the understanding between low nucleic acid content (LNA) bacteria and filter bacteria. [methods] flow cytometry, denaturing gradient gel electrophoresis (DGGE) and statistical analysis were used to study the relationship between bacterial communities and bacteria in three typical freshwater environments. [results] there was a good correlation between the number of LNA bacteria and the number of filter bacteria. The filterability of LNA bacteria was not only related to the size of bacteria, but also to the number of bacteria. There was a negative correlation between bacterial community and the specific gravity of LNA bacteria, but a positive correlation with HNA bacteria. [conclusion] 0.45 渭 m membrane filtration has a strong screening effect on LNA bacteria in water sample microbial community. Bacterial community tissue is closely related to the gene content determined by flow cytometry.
【作者单位】： 南开大学环境科学与工程学院环境污染过程与基准教育部重点实验室天津市城市生态环境修复与污染防治重点实验室;
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