不同类型脂肪酸对HepG2细胞胰岛素抵抗的作用及Toll样受体4表达的影响

发布时间：2018-05-03 16:32

本文选题：脂肪酸 + 胰岛素抵抗　；参考：《卫生研究》2017年06期

【摘要】：目的观察不同类型脂肪酸对HepG2细胞胰岛素抵抗的作用及探讨可能的作用机制。方法 250μmol/L的棕榈酸(PA)、花生四烯酸(AA)以及二十碳五烯酸(EPA)分别与HepG2细胞共同培养24 h,葡萄糖氧化酶法测定细胞上清液中葡萄糖含量,酶联免疫吸附法测定炎性因子白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量,以及实时聚合酶链反应(Real-time PCR)检测Toll样受体4(TLR4)mRNA的表达。结果 PA组培养液中葡萄糖含量显著高于空白对照组(P0.05),EPA组可增加葡萄糖消耗量,与PA组及AA组比较差异具有统计学意义(P0.05)。各脂肪酸干预组细胞上清液中炎性因子IL-6和TNF-α含量均增加,EPA组增加量最低,PA组和AA组上清液中IL-6含量与空白组比较差异有统计学意义(P0.05)。各脂肪酸干预组与空白组比较TLR4 mRNA表达量均增加(P均0.05),EPA组增加量最低。结论高浓度PA降低了HepG2细胞对胰岛素的敏感性,能够引起胰岛素抵抗,AA具有降低胰岛素敏感性的趋势,而EPA则具有增加胰岛素敏感性的趋势,TLR4信号转导通路及其启动的炎症反应可能是其重要的作用机制。
[Abstract]:Objective to observe the effects of different fatty acids on insulin resistance in HepG2 cells and to explore the possible mechanism. Methods 渭 mol/L palmitic acid (PAA), arachidonic acid (AA) and eicosapentaenoic acid (EPA) were co-cultured with HepG2 cells for 24 hours. Glucose content in the supernatant was determined by glucose oxidase method. The levels of interleukin-6 (IL-6) and tumor necrosis factor- 伪 (TNF- 伪) were measured by enzyme-linked immunosorbent assay (Elisa), and the expression of Toll like receptor (4(TLR4)mRNA) was detected by real-time polymerase chain reaction (PCR). Results the glucose content in the culture medium of PA group was significantly higher than that of the control group (P 0.05). The glucose consumption in PA group was significantly higher than that in PA group and AA group, and there was a significant difference between PA group and AA group. The content of inflammatory factor IL-6 and TNF- 伪 in the supernatant of each fatty acid intervention group was significantly higher than that in the control group. The content of IL-6 in the supernatant of PA group and AA group was significantly higher than that in the control group. The increase of TLR4 mRNA expression in each fatty acid intervention group was the lowest compared with that in the blank group. Conclusion High concentration of PA can decrease the insulin sensitivity of HepG2 cells and induce insulin resistance. EPA has a tendency to increase insulin sensitivity. TLR4 signal transduction pathway and its initiated inflammatory response may be the important mechanism of TLR4 signal transduction pathway.
【作者单位】：南京中医药大学第二临床医学院;
【基金】：江苏省高校自然科学研究面上项目(No.15KJB330004)
【分类号】：R151

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