广州市售肉类食品源大肠埃希菌耐药性分析

发布时间：2018-05-13 05:05

本文选题：大肠埃希菌 + 肉类食品源　；参考：《华南农业大学学报》2017年02期

【摘要】：【目的】了解广州市售肉类食品源大肠埃希菌的污染和耐药情况。【方法】自2011年7月至8月,从广州市各大农贸市场和超市采集市售肉类样品310份,其中猪肉253份,鸡肉57份。采用选择性培养基分离鉴定大肠埃希菌Escherichia coli,琼脂稀释法测定大肠埃希菌对19种抗菌药物的最小抑菌浓度(MIC)。【结果】310份样品中共分离到213株大肠埃希菌,分离率为68.7%,其中猪肉源177株,鸡肉源36株。受试菌株对复方新诺明、链霉素和四环素的耐药率超过75.0%,对氨苄西林、萘啶酸、氯霉素、新霉素、氟苯尼考、磷霉素、环丙沙星、安普霉素、恩诺沙星、庆大霉素和头孢唑啉的耐药率为10.0%~60.0%,而对头孢西丁、头孢曲松、头孢他啶、黏菌素和阿米卡星表现较为敏感,耐药率小于5.0%。鸡肉源大肠埃希菌对头孢唑啉、头孢西丁、头孢曲松、头孢他啶和磷霉素的耐药率均高于猪肉源大肠埃希菌,差异极显著(P0.01)。213株大肠埃希菌中82.2%为多重耐药菌。【结论】广州市售肉类食品存在较为严重的多重耐药大肠埃希菌污染,且鸡肉源大肠埃希菌耐药性较猪肉源大肠埃希菌严重。
[Abstract]:[objective] to investigate the contamination and drug resistance of Escherichia coli from meat food sources in Guangzhou. [methods] from July to August 2011, 310 meat samples were collected from farmers' markets and supermarkets in Guangzhou, including 253 pork samples. Chicken 57. Escherichia coli was isolated and identified by selective culture medium, and the minimal inhibitory concentration of Escherichia coli to 19 antimicrobial agents was determined by Agar dilution method. [results] 213strains of Escherichia coli were isolated from 310 samples. The isolation rate was 68.7, of which 177 were from pork and 36 from chicken. The resistance rate of the tested strains to compound sulfamethoxine, streptomycin and tetracycline was more than 75.0, and they were resistant to ampicillin, naphthalic acid, chloramphenicol, neomycin, florfenicol, fosfomycin, ciprofloxacin, ampicillin, enrofloxacin, The drug resistance rates of gentamicin and cefazolin were 10.0 and 60.0, but were sensitive to cefxitin, ceftriaxone, ceftazidime, mucin and amikacin, and the resistance rate was less than 5.0. The resistance rates of Escherichia coli from chicken to cefazolin, cefxitin, ceftriaxone, ceftazidime and fosfomycin were higher than those of pork-derived Escherichia coli. 82.2% of the strains of Escherichia coli were multidrug resistant. [conclusion] there was a serious contamination of multidrug resistant Escherichia coli in meat products sold in Guangzhou, and the resistance of chicken origin Escherichia coli was more serious than that of pork origin Escherichia coli.
【作者单位】：华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室;佛山市正典生物技术有限公司;
【分类号】：S852.61

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