全谷豆复合包膳食纤维的分析及其对大鼠脂代谢紊乱及SREBP-1c调控的影响
发布时间：2018-09-17 14:13
【摘要】：目的：(1)测定并比较个吞豆复合包和全谷物玉米中总膳食纤维、可溶性膳食纤维和不可溶性膳食纤维的含量。确定提取膳食纤维的最佳工艺,并且测定提取所得膳食纤维的理化性质。(2)观察并比较全谷豆复合包、全谷豆复合包膳食纤维和全谷物全谷物玉米膳食纤维对于高脂高胆固醇饲料诱导脂代谢紊乱大鼠血脂及其肝脏脂肪酸合成酶(fatty acid synthase,FAS)活性的影响,并观察其对大鼠肝组织固醇调节元件结合蛋自-1c (sterol regulatory element binding protein-1c, SREBP-1c) mRNA表达的调控作用。 方法：(1)采用酶-化学法提取个谷豆复合包和全谷物玉米中总膳食纤维,采用酶-重量法测定其中膳食纤维的含量。(2)50只SD大鼠适应性喂养1周后,断尾取血,收集血清,测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白固醇(HDL-C)和空腹血糖(FBG)。并以总胆固醇(TC)水平,随机分成阴性组、高脂模型组、全谷豆复合包组、全谷豆复合包膳食纤维组和全谷物玉米膳食纤维组。参照AIN-93M配方配制阴性组饲料,在此基础上增加10%饱和脂肪酸和1.5%胆固醇制成高脂模型组饲料、全谷豆复合包组饲料、全谷豆复合包膳食纤维组饲料和全谷物玉米膳食纤维组饲料。(3)连续喂养8周后,测定各组大鼠TC、TG、FBG和HDL-C。动物处死后,取-叶肝脏以10%甲醛固定,HE染色,光镜下观察肝脏脂肪变性情况。(4)采用双抗体两步夹心酶联免疫吸附法(Elisa)测定各组大鼠肝脏脂肪酸合成酶(FAS)活性。(5)应用半定最逆转录-聚合酶链反应(RT-PCR)方法测定各组大鼠肝脏SREBP-1c的mRNA。 结果：(1)每100g全谷豆复合包平均可以提取得到14.58膳食纤维；每100g个谷物玉米平均可以提取得到5g膳食纤维。(2)每100g个谷豆复合包含总膳食纤维(total dietary fiber, TDF)15.3g、不可溶性膳食纤维(insoluble dietary fiber, IDF)13.97g和可溶性膳食纤维(soluble dietary fiber, SDF)1.33g;每100g全谷物玉米粉中含TDF9.1g、IDF8.10g、 SDF0.996g。(3)全谷豆复合包DF和全谷物玉米DF的膨胀力分别为：10.19ml/g和8.1ml/g;持水力：8.83g/g和7.7g/g;不饱和脂肪的吸附力：1.64g/g和1.05g/g;饱和脂肪的吸附力：1.89g/g和1.2g/g。(4)动物实验结果表明：与阴性对照组织相比,高脂模型的大鼠血洁TCTG、FBG和LDL-C水平显著升高(p0.05)；万高脂模型组相比,全谷豆复合包药组、全豆复合包膳食纤维组和全谷物玉米膳食纤维组实验大鼠血清rC、TG、FBG和LDL-C水平显著降低(P0.05);全谷豆复合包组、全谷豆复合包膳食纤维组和全谷物玉米膳食纤维组实验大鼠血清HDL-C水平显著高于高脂模型组；(5)实验大鼠HE切片结果显示,全谷豆复合包组、全谷豆复合包膳食纤维组和全谷物玉米膳食纤维组可以显著减轻大鼠肝脏脂肪变性的程度。(6)全谷豆复合包组、全谷豆复合包膳食纤维组和全谷物玉米膳食纤维组大鼠肝脏FAS活性显著低于高脂模型组；大鼠肝脏组织的SREBP-lc的表达显著降低。 结论：(1)全谷豆复合包中含丰富的膳食纤维,约是全谷物玉米的1.7倍,且其膳食纤维的得率约是全谷物玉米膳食纤维5倍,主要可能原因是全谷物玉米中含大量的淀粉较难去除。(2)用酶-化学法提取的DF理化性质较好,提取得率较高,并且其纯度较高。(3)全谷豆复合包膳食纤维具有改善脂代谢紊乱大鼠的血脂水平。(4)全谷豆复合包膳食纤维可以降低FAS的活性,并且可以降低实验大鼠肝脏SREBP-lc的表达水平。
[Abstract]:OBJECTIVE: (1) To determine and compare the contents of total dietary fiber, soluble dietary fiber and insoluble dietary fiber in whole-grain corn and whole-bean composite package, determine the optimum extraction technology of dietary fiber, and determine the physicochemical properties of the extracted dietary fiber. (2) To observe and compare the dietary fiber of whole-grain bean composite package and whole-grain bean composite package. Effects of whole-grain corn dietary fiber and whole-grain corn dietary fiber on serum lipids and fatty acid synthase (FAS) activity in liver of rats with lipid metabolism disorder induced by high-fat and high-cholesterol diet, and its effect on sterol regulatory element binding protein-1c (SREBP-1c) mRNA in rat liver tissue were observed. Regulation of expression.
METHODS: (1) Total dietary fiber was extracted by enzyme-chemical method from whole-grain corn and whole-grain corn, and the content of dietary fiber was determined by enzyme-gravimetric method. (2) Blood samples were collected from tail of 50 SD rats after one week of adaptive feeding, and total cholesterol (TC), triglyceride (TG), high-density lipoprotein sterol (HDL-C) and fasting blood glucose (FB) were measured. G) and total cholesterol (TC) levels were randomly divided into negative group, high-fat model group, whole-grain-soybean composite package group, whole-grain-soybean composite package dietary fiber group and whole-grain corn dietary fiber group. (3) After 8 weeks of continuous feeding, TC, TG, FBG and HDL-C of rats in each group were determined. The liver was fixed with 10% formaldehyde and stained with HE. The fatty degeneration of liver was observed under light microscope. (5) The mRNA of SREBP-1c in rat liver was determined by semi-definite reverse transcription-polymerase chain reaction (RT-PCR).
Results: (1) The average dietary fiber was 14.58 per 100 g of whole grains and beans, and 5 g per 100 g of corn. (2) The total dietary fiber (TDF), insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) were 15.3 g, 13.97 g and 100 g of whole grains and beans respectively. Soluble dietary fiber (SDF) 1.33 g; TDF 9.1 g, IDF 8.10 g, SDF 0.996 g per 100 g whole grain corn flour; swelling power of whole grain and soybean composite coating DF and whole grain corn DF were 10.19 ml/g and 8.1 ml/g; water holding capacity: 8.83 g/g and 7.7 g/g; adsorption capacity of unsaturated fat: 1.64 g/g and 1.05 g/g; adsorption capacity of saturated fat: 1.89g/g and 8.89 g/g, respectively. The results of 1.2g/g. (4) Animal experiment showed that the levels of TCTG, FBG and LDL-C in serum of rats with hyperlipidemia model were significantly higher than those of rats with negative control group (p0.05); compared with those of rats with hyperlipidemia model, the levels of rC, TG, FBG and LDL-C in serum of rats with whole-grain-soybean composite dietary fiber group, whole-bean composite dietary fiber group and whole-grain corn dietary fiber group were significantly higher. The serum HDL-C levels of rats in the whole-grain-soybean dietary fiber group, the whole-grain-soybean dietary fiber group and the whole-grain-corn dietary fiber group were significantly higher than those in the high-fat model group. (5) HE slices of the experimental rats showed that the whole-grain-soybean dietary fiber group, the whole-grain-soybean dietary fiber group and the whole-grain-corn dietary fiber group could be significantly higher than the whole-grain-soybean dietary fiber group. (6) The activity of FAS in rat liver was significantly lower than that in the high-fat model group, and the expression of SREBP-lc in rat liver was significantly lower in the whole-grain-soybean complex group, the whole-grain-soybean complex dietary fiber group and the whole-grain-corn dietary fiber group.
CONCLUSIONS: (1) The whole grain and soybean complex package contains about 1.7 times as much dietary fiber as whole grain corn, and the yield of dietary fiber is about 5 times as much as that of whole grain corn. The main reason may be that it is difficult to remove starch from whole grain corn. (2) The physicochemical properties of DF extracted by enzyme-chemical method are better, and the extraction rate is higher. (3) Whole-grain-bean dietary fiber could improve the blood lipid level of rats with lipid metabolism disorder. (4) Whole-grain-bean dietary fiber could decrease the activity of FAS and the expression of SREBP-lc in the liver of experimental rats.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151
本文编号：2246182
[Abstract]:OBJECTIVE: (1) To determine and compare the contents of total dietary fiber, soluble dietary fiber and insoluble dietary fiber in whole-grain corn and whole-bean composite package, determine the optimum extraction technology of dietary fiber, and determine the physicochemical properties of the extracted dietary fiber. (2) To observe and compare the dietary fiber of whole-grain bean composite package and whole-grain bean composite package. Effects of whole-grain corn dietary fiber and whole-grain corn dietary fiber on serum lipids and fatty acid synthase (FAS) activity in liver of rats with lipid metabolism disorder induced by high-fat and high-cholesterol diet, and its effect on sterol regulatory element binding protein-1c (SREBP-1c) mRNA in rat liver tissue were observed. Regulation of expression.
METHODS: (1) Total dietary fiber was extracted by enzyme-chemical method from whole-grain corn and whole-grain corn, and the content of dietary fiber was determined by enzyme-gravimetric method. (2) Blood samples were collected from tail of 50 SD rats after one week of adaptive feeding, and total cholesterol (TC), triglyceride (TG), high-density lipoprotein sterol (HDL-C) and fasting blood glucose (FB) were measured. G) and total cholesterol (TC) levels were randomly divided into negative group, high-fat model group, whole-grain-soybean composite package group, whole-grain-soybean composite package dietary fiber group and whole-grain corn dietary fiber group. (3) After 8 weeks of continuous feeding, TC, TG, FBG and HDL-C of rats in each group were determined. The liver was fixed with 10% formaldehyde and stained with HE. The fatty degeneration of liver was observed under light microscope. (5) The mRNA of SREBP-1c in rat liver was determined by semi-definite reverse transcription-polymerase chain reaction (RT-PCR).
Results: (1) The average dietary fiber was 14.58 per 100 g of whole grains and beans, and 5 g per 100 g of corn. (2) The total dietary fiber (TDF), insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) were 15.3 g, 13.97 g and 100 g of whole grains and beans respectively. Soluble dietary fiber (SDF) 1.33 g; TDF 9.1 g, IDF 8.10 g, SDF 0.996 g per 100 g whole grain corn flour; swelling power of whole grain and soybean composite coating DF and whole grain corn DF were 10.19 ml/g and 8.1 ml/g; water holding capacity: 8.83 g/g and 7.7 g/g; adsorption capacity of unsaturated fat: 1.64 g/g and 1.05 g/g; adsorption capacity of saturated fat: 1.89g/g and 8.89 g/g, respectively. The results of 1.2g/g. (4) Animal experiment showed that the levels of TCTG, FBG and LDL-C in serum of rats with hyperlipidemia model were significantly higher than those of rats with negative control group (p0.05); compared with those of rats with hyperlipidemia model, the levels of rC, TG, FBG and LDL-C in serum of rats with whole-grain-soybean composite dietary fiber group, whole-bean composite dietary fiber group and whole-grain corn dietary fiber group were significantly higher. The serum HDL-C levels of rats in the whole-grain-soybean dietary fiber group, the whole-grain-soybean dietary fiber group and the whole-grain-corn dietary fiber group were significantly higher than those in the high-fat model group. (5) HE slices of the experimental rats showed that the whole-grain-soybean dietary fiber group, the whole-grain-soybean dietary fiber group and the whole-grain-corn dietary fiber group could be significantly higher than the whole-grain-soybean dietary fiber group. (6) The activity of FAS in rat liver was significantly lower than that in the high-fat model group, and the expression of SREBP-lc in rat liver was significantly lower in the whole-grain-soybean complex group, the whole-grain-soybean complex dietary fiber group and the whole-grain-corn dietary fiber group.
CONCLUSIONS: (1) The whole grain and soybean complex package contains about 1.7 times as much dietary fiber as whole grain corn, and the yield of dietary fiber is about 5 times as much as that of whole grain corn. The main reason may be that it is difficult to remove starch from whole grain corn. (2) The physicochemical properties of DF extracted by enzyme-chemical method are better, and the extraction rate is higher. (3) Whole-grain-bean dietary fiber could improve the blood lipid level of rats with lipid metabolism disorder. (4) Whole-grain-bean dietary fiber could decrease the activity of FAS and the expression of SREBP-lc in the liver of experimental rats.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151
【参考文献】
相关期刊论文 前10条
1 艾正琳;陈东风;;SREBP-1c在大鼠非酒精性脂肪性肝病中的表达及意义[J];第三军医大学学报;2006年10期
2 童国玉;固醇调节元件结合蛋白1c的研究进展[J];国外医学.内分泌学分册;2002年05期
3 张鑫毅;孙建琴;;膳食纤维与健康[J];国外医学(卫生学分册);2007年03期
4 潘英明,梁英,王恒山,葛春玉,袁伟恩,银秋兰;从罗汉果渣中提取水不溶性膳食纤维的研究[J];广西植物;2003年04期
5 曹银;杨芳;周露;;碱法与酶法提取大麦糟膳食纤维的比较[J];现代食品科技;2011年03期
6 钟节鸣;俞敏;刘立群;陈雅萍;胡如英;龚巍巍;;浙江省居民膳食营养摄入状况研究[J];疾病监测;2006年12期
7 吴卫国,郭时印,周虹;麦麸膳食纤维的制备、性质与应用[J];粮食与饲料工业;1998年11期
8 王卫东;徐婷娟;徐维平;胡世莲;;心脑血管疾病危险因子的Meta分析[J];中国临床保健杂志;2010年05期
9 吕金顺,杨声,王小芳,左国防;马铃薯渣制膳食纤维的工艺研究[J];中国马铃薯;2001年06期
10 朱毓芬;吴保锋;田庆生;;膳食纤维的性质与作用[J];内蒙古科技与经济;2007年19期
相关硕士学位论文 前1条
1 郑红艳;小米麸皮膳食纤维的提取及成分和功能性质研究[D];西南大学;2010年
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