莱菔硫烷对染镉小鼠睾丸间质细胞抗氧化功能影响的研究
发布时间：2018-10-14 15:40
【摘要】：试验旨在研究莱菔硫烷(SFN)对染镉(Cd)小鼠睾丸间质细胞(TM3细胞)抗氧化应激能力的影响。向TM3细胞培养液中添加Cd和SFN,分别测定Cd的半数抑制浓度(IC50)和SFN的安全剂量范围,建立试验模型,通过检测TM3细胞的相对存活率、乳酸脱氢酶(LDH)活性及细胞抗氧化水平,判定SFN对Cd诱导TM3细胞毒性的影响。结果显示:1随着Cd浓度的增加,TM3细胞的相对存活率降低,Cd对体外培养TM3细胞的IC50为51.4μmol/L;SFN在一定范围内对细胞存活有促进作用,但超过范围后具有毒性,且浓度越大毒性越大,本试验条件下选择SFN浓度为2.5、5和10μmol/L。2与对照组相比,Cd组TM3细胞的T-SOD、GSH-Px活性及GSH含量显著或极显著降低(P0.05;P0.01),LDH活性、MDA含量升高;SFN组LDH活性、MDA含量降低,GSH含量、T-SOD活性及GSH-Px活力升高;与Cd组相比,Cd+SFN组GSH含量、T-SOD活性及GSH-Px活力显著或极显著升高(P0.05;P0.01);LDH活性、MDA含量则降低,表明SFN具有缓解染Cd诱导TM3细胞毒性的作用。
[Abstract]:The aim of this study was to investigate the effect of sulforaphane (SFN) on antioxidant stress of testis interstitial cells (TM3 cells) in cadmium-exposed (Cd) mice. The half inhibitory concentration (IC50) of Cd and the safe dose range of SFN were measured by adding Cd and SFN, to the culture medium of TM3 cells, and the experimental model was established. The relative survival rate of TM3 cells, the activity of lactate dehydrogenase (LDH) and the antioxidant level of TM3 cells were measured. To evaluate the effect of SFN on TM3 cell toxicity induced by Cd. The results showed that: 1 with the increase of Cd concentration, the relative survival rate of TM3 cells decreased. Cd promoted the survival of TM3 cells in a range of 51.4 渭 mol/L;SFN, but beyond the range was toxic, and the greater the concentration was, the greater the toxicity was. Compared with the control group, the activity of T-SODD-GSH-Px and the content of GSH in TM3 cells of Cd group were significantly or extremely decreased (P0.05P0.01), LDH activity, MDA content increased, LDH activity, MDA content decreased, GSH content, T-SOD activity and GSH-Px activity increased in SFN group compared with the control group, the concentration of SFN was 2.5 渭 mol/L.2 and 10 渭 mol/L.2, respectively). Compared with Cd group, GSH content, T-SOD activity and GSH-Px activity in, Cd SFN group were significantly increased (P0.05 + P0.01); LDH activity, MDA content was decreased, indicating that SFN could alleviate TM3 cytotoxicity induced by Cd.
【作者单位】： 沈阳农业大学畜牧兽医学院;
【基金】：沈阳农业大学博士后基金
【分类号】：R114
[Abstract]:The aim of this study was to investigate the effect of sulforaphane (SFN) on antioxidant stress of testis interstitial cells (TM3 cells) in cadmium-exposed (Cd) mice. The half inhibitory concentration (IC50) of Cd and the safe dose range of SFN were measured by adding Cd and SFN, to the culture medium of TM3 cells, and the experimental model was established. The relative survival rate of TM3 cells, the activity of lactate dehydrogenase (LDH) and the antioxidant level of TM3 cells were measured. To evaluate the effect of SFN on TM3 cell toxicity induced by Cd. The results showed that: 1 with the increase of Cd concentration, the relative survival rate of TM3 cells decreased. Cd promoted the survival of TM3 cells in a range of 51.4 渭 mol/L;SFN, but beyond the range was toxic, and the greater the concentration was, the greater the toxicity was. Compared with the control group, the activity of T-SODD-GSH-Px and the content of GSH in TM3 cells of Cd group were significantly or extremely decreased (P0.05P0.01), LDH activity, MDA content increased, LDH activity, MDA content decreased, GSH content, T-SOD activity and GSH-Px activity increased in SFN group compared with the control group, the concentration of SFN was 2.5 渭 mol/L.2 and 10 渭 mol/L.2, respectively). Compared with Cd group, GSH content, T-SOD activity and GSH-Px activity in, Cd SFN group were significantly increased (P0.05 + P0.01); LDH activity, MDA content was decreased, indicating that SFN could alleviate TM3 cytotoxicity induced by Cd.
【作者单位】： 沈阳农业大学畜牧兽医学院;
【基金】：沈阳农业大学博士后基金
【分类号】：R114
【参考文献】
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1 韩建鑫;何剑斌;高锋;杨淑华;梁甜甜;董双;张q，
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