昼夜节律及饮食对主被动吸烟者尿Cotinine浓度的影响

发布时间：2018-10-22 07:14

【摘要】：目的：研究昼夜节律和就餐行为对于吸烟者及被动暴露人群尿液可的宁浓度的影响,研究生活习性对于体内尼古丁代谢速率的影响。方法：选取32对夫妇,每组分别在晨间6-7点及下午5-6点集中于固定的面积为25m2的普通民居,男性吸烟者在1小时内共吸烟4支,女性为被动吸烟者,共同暴露1小时,提取试验后1小时、6小时尿样,加餐组在实验第4小时加入正常餐饮一次,最后尿样以ELISA法测定各个时间点的Cotinine浓度,分析Cotinine整体的衰减趋势,日间与夜间的5小时范围内的Cotinine衰减幅度差异,未加餐与夹杂进食行为的5小时范围内的Cotinine衰减幅度差异。结果：32位男性7点样本分别为757.4±207.8ng/ml、729.1±173.6ng/ml,13点样本为562.8±162.0ng/ml、458.3±170.8ng/ml(加餐)、19点样本为684.3±131.5ng/ml、647.8±205.2ng/ml,24点样本为589.6±202.6ng/ml、484.2±143.8ng/ml(加餐)。所有组内的2时间点Cotinine浓度进行独立T检验,P值均0.05,比较均值后发现,随时间都呈现衰减。晨间5小时衰减值均值为647.885ng/ml,标准差为205.26ng/ml,夜间5小时衰减值均值为484.254rig/ml,标准差为143.858ng/ml两组数据进行独立T检验,方差不等,P0.05,有统计学差异。晨间未加餐5小时衰减值均值为194.6322ng/ml,标准差为54.406ng/ml,晨间加餐5小时衰减值均值为270.8161ng/ml,标准差为47.26ng/ml,两组数据进行独立T检验,方差不等,P0.05,有统计学差异。夜间未加餐组5小时衰减值均值为94.719ng/ml,标准差为95.16342ng/ml,夜间加餐组5小时衰减值均值为163.631ng/ml,标准差为65.386ng/ml,两组进行独立T检验,方差不等,P0.05,有统计学差异,夜间加餐组5小时衰减值夜间未加餐组5小时衰减值。 32位女性被动吸烟者的浓度分别为7点：45.21±26.1ng/ml、45.8±14.7ng/ml、13点:32.9±19.6ng/ml、31.7±21.4ng/m1(加餐)、19点：47.4±25.2ng/ml、38.5±21.0ng/ml、24点40.1±21.8ng/ml、24.2±5.3ng/ml(加餐)。未加餐晨间衰减值均值为38.559ng/ml,标准差为21.043ng/ml,未加餐夜间衰减值均值为24.276ng/ml,标准差为15.329ng/ml,两组进行独立T检验,方差不相等,P0.05,有统计学差异,晨间未加餐衰减值未加餐夜间衰减值。晨间未加餐衰减值均值为12.2857ng/ml,标准差为7.333ng/ml,晨间加餐衰减值均值为14.0922ng/ml,标准差为9.745Ong/ml,两组进行独立T检验,方差相等,P0.05,无统计学差异,晨间未加餐衰减值与晨间加餐衰减值无统计学差异。结论：主动吸烟者晨间吸烟可导致日间尼古丁代谢呈现较快水平,可影响日间体内循环可的宁处于相对高水平,易导致对后续尼古丁的渴求行为增加。被动吸烟者亦受昼夜节律影响,但不明显,整体绝对值变化不大,可能系本身体内循环可的宁水平较低有关,进食行为对于尼古丁代谢有促进作用,短时间内可加快Cotinine的代谢。
[Abstract]:Aim: to study the effects of circadian rhythm and eating behavior on the concentration of Karenin in urine of smokers and passive exposed people, and to study the effect of life habits on the metabolic rate of nicotine in vivo. Methods: 32 couples in each group were divided into two groups in the morning at 6: 7 and 5 to 6: 00 in the morning respectively. Male smokers smoked 4 cigarettes in one hour, and women were passive smokers who were exposed for 1 hour. The urine samples were extracted 1 hour and 6 hours after the experiment. The added meal group added normal food once in the 4th hour of the experiment. Finally, the concentration of Cotinine at each time point was measured by ELISA method, and the overall attenuation trend of Cotinine was analyzed. The range of Cotinine attenuation was different between daytime and night within 5 hours, and that between non-meal and inclusion eating within 5 hours. Results: in 32 males, 729.1 卤173.6ng / ml of 729.1 卤173.6ng / ml / min were 562.8 卤162.0ng / ml / ml 458.3 卤170.8ng/ml, and 684.3 卤131.5ng / ml / ml 647.8 卤205.2ngml / ml 24 points were 589.6 卤202.6ng / ml / min and 589.6 卤202.6ng / ml / min = 589.6 卤202.6ng / ml / ml = 484.2 卤143.8ng/ml, respectively. The Cotinine concentration in all groups was tested by independent T test at 2 time points, P value was 0.05. After comparing the mean value, it was found that all of them showed attenuation with time. The average attenuation value of 5 hours in the morning was 647.885ng / ml, the standard deviation was 205.26ng / ml, and the mean value of 5-hour attenuation value at night was 484.254rigp / ml. The standard deviation of the two groups of data was tested by independent T test, the variance was not equal to that of the two groups (P0.05, P 0.05). The average attenuation value of 5 hours in the morning was 194.6322 ng / ml, the standard deviation was 54.406 ng / ml, the average attenuation value of 5 hours was 270.8161 ng / ml, and the standard deviation was 47.26 ng / ml. The two groups of data were tested by independent T test, the variance was not equal to that of P0.05, there was statistical difference between the two groups. The mean value of 5 hours attenuation was 94.719 ng / ml and the standard deviation was 95.16342ng / ml in the nocturnal group, while the mean value of the 5-hour attenuation value was 163.631 ng / ml and the standard deviation was 65.386 ng / ml in the night plus meal group. The two groups were tested by independent T test, the variance was different (P0.05), there was statistical difference between the two groups. Attenuation value of 5 hours in nocturnal plus meal group. The concentration of 32 female passive smokers were 45.21 卤26.1ngml / ml 45.8 卤14.7 ng / ml 13 points: 32.9 卤19.6nggml / ml / ml 31.7 卤21.4ng/m1 respectively, 19:00: 47.4 卤25.2ngml / ml 38.5 卤21.0ngml / ml 24: 40.1 卤21.8ngml-1 卤24.2 卤5.3ng/ml. The average attenuation value of non-meal morning was 38.559 ng / ml, the standard deviation was 21.043 ng / ml, the mean value of nocturnal attenuation was 24.276 ng / ml and the standard deviation was 15.329 ng / ml. The independent T test showed that the variance was not equal and the variance was not equal (P0.05), there was statistical difference between the two groups. The average attenuation value of non-meal in the morning was 12.2857ng / ml, the standard deviation was 7.333ng / ml, the average attenuation value of the morning meal was 14.0922ng / ml, and the standard deviation was 9.745Ong / ml. The two groups were tested by independent T test, the variance was equal, and there was no statistical difference between the two groups (P0.05). There was no statistical difference between the attenuation value of morning meal and that of morning meal. Conclusion: morning smoking in active smokers can lead to a faster level of day nicotine metabolism, which can affect the higher level of circulatory activity in the daytime, and may lead to an increase of nicotine craving behavior. Passive smokers were also influenced by circadian rhythm, but not obviously. The total absolute value was not changed significantly, which might be related to the lower level of Kokonin in the body. Eating behavior could promote nicotine metabolism and accelerate the metabolism of Cotinine in a short period of time.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R163

【相似文献】