环境剂量的双酚A暴露对雄性小鼠生殖功能的影响

发布时间：2018-11-09 11:13

【摘要】：目的:研究环境剂量的双酚A(BPA)暴露对雄性小鼠生殖功能的影响及可能的分子机制。方法:84只3周龄C57BL/6J雄性小鼠按体质量随机分为3组,分别为5μg/kg BPA组、50μg/kg BPA组、乙醇溶剂对照组,每组28只。每日灌胃给药1次,连续给药5周后,检测生殖器官脏器指数、附睾尾精子数量、睾丸组织学形态;同期与正常雌性小鼠配种检测雄鼠的生育力。应用MBDq PCR方法检测睾丸中Tnnt2、Tectb基因的甲基化水平变化。结果:1 BPA暴露组与对照组间的生殖系统脏器指数无统计学差异(P0.05)。2 50μg/kg BPA暴露组附睾尾精子数量下降20.1%;睾丸曲细精管管腔中有生精细胞脱落等病理现象。3 BPA暴露组与对照组间的生育率无统计学差异(P0.05),但50μg/kg BPA暴露组每胎平均产仔数下降。4 BPA组睾丸中Tnnt2、Tectb基因的甲基化水平下降(P0.01)。结论:环境剂量的BPA暴露对雄鼠生殖器官脏器指数没有影响,50μg/kg BPA能引起小鼠生精功能下降,睾丸Tnnt2、Tectb基因的甲基化水平下降。
[Abstract]:Aim: to study the effect of environmental dose of bisphenol A (BPA) exposure on reproductive function of male mice and its possible molecular mechanism. Methods: 84 3-week-old C57BL/6J male mice were randomly divided into 3 groups according to their body weight: 5 渭 g/kg BPA group, 50 渭 g/kg BPA group and ethanol solvent control group. The organ index of reproductive organs, sperm count of epididymis tail and testicular histology were measured after 5 weeks of continuous administration by gavage, and the fertility of male mice was detected by mating with normal female mice at the same time. MBDq PCR method was used to detect the methylation level of Tnnt2,Tectb gene in testis. Results: 1 there was no significant difference in organ index of reproductive system between BPA exposure group and control group (P0.05). The sperm count of epididymal tail decreased by 20.1in 250 渭 g/kg BPA exposure group. There was no significant difference in fertility between the BPA exposure group and the control group (P0.05), but the average litter size per fetus in the 50 渭 g/kg BPA exposure group decreased. 4 Tnnt2, in the testis of the BPA group. The methylation level of Tectb gene decreased (P0.01). Conclusion: environmental dose of BPA has no effect on organ index of male reproductive organs. 50 渭 g/kg BPA can decrease spermatogenic function and decrease the methylation level of Tnnt2,Tectb gene in testis.
【作者单位】：吉林大学白求恩医学院病理生理学教研室;国家卫生与计划生育药具重点实验室上海市计划生育科学研究所;
【基金】：国家自然科学基金(基金编号:81270760) 上海市自然科学基金(编号:14ZR1435400)资助项目
【分类号】：R114

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