实时荧光PCR法同时检测食物中大豆和芹菜致敏原成分

发布时间：2018-11-10 08:17

【摘要】：大豆、芹菜等食品原料是一些特殊人群的致敏原。根据大豆atp A基因和芹菜mtd基因设计特异性引物,利用不同荧光素标记的Taq Man探针,建立了一种实时荧光PCR检测方法,可同时检测食物中大豆和芹菜致敏原成分。分别以大豆、芹菜、大米、小麦、大麦、花生、芝麻、玉米、马铃薯、蕃茄、核桃、开心果、腰果、葵花籽、杏仁、苹果、梨、草莓、猪肉、牛肉、羊肉及鱼肉等材料的基因组DNA作为模板,进行PCR扩增实验,结果发现该方法仅能特异性扩增大豆和芹菜致敏原成分。灵敏度测试结果表明大豆和芹菜成分的检出限均达0.01%。因此,本方法可以作为同时检测食品中大豆和芹菜致敏原成分的特异性方法。
[Abstract]:Soybean, celery and other food raw materials are some special people allergen. Based on the design of specific primers of soybean atp A gene and celery mtd gene, a real-time fluorescent PCR detection method was developed using Taq Man probes labeled with different fluorescein, which can be used to detect the allergen components of soybean and celery simultaneously. With soybeans, celery, rice, wheat, barley, peanuts, sesame, corn, potatoes, tomatoes, walnuts, pistachios, cashews, sunflower seeds, almonds, apples, pears, strawberries, pork, beef, The genomic DNA of mutton and fish was used as template for PCR amplification. The results showed that this method could only amplify the allergen components of soybean and celery. The sensitivity test showed that the detection limit of soybean and celery components was 0.01. Therefore, this method can be used as a specific method for simultaneous detection of soybean and celery allergens.
【作者单位】：国家农副加工食品质量监督检验中心安徽省食品药品检验研究院;
【基金】：安徽省质监局科研项目(13zj37003)
【分类号】：R155.5

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