RT-PCR检测食品中诺如病毒检测研究进展
发布时间：2018-11-14 08:13
【摘要】：诺如病毒是一种通过食品传染进入人体导致人体急性肠胃炎的杯状病毒。诺如病毒的发病范围越来越广,造成的危害也越来越大。但由于诺如病毒富集及其核酸提取的困难,而且目前缺乏标准有效的诺如病毒检测方法,很难准确地检测到诺如病毒在食品中的存在。目前,荧光定量PCR由于其高灵敏度和特异性被广泛应用于食品中诺如病毒的检测,能有效预防食品中诺如病毒对人体的危害。因此,对诺如病毒的富集、核酸提取方法、诺如病毒的RT-PCR检测方法进行了综述,为控制食品中诺如病毒对人体的危害奠定基础。
[Abstract]:Norovirus is a goblet virus that infects the body through food and causes acute gastroenteritis. Norovirus is more and more widespread, causing more and more harm. However, it is difficult to detect the presence of Norovirus in food precisely because of the difficulty of enrichment and nucleic acid extraction of Norovirus and the lack of standard and effective methods for detection of Norovirus. At present, fluorescent quantitative PCR is widely used in the detection of Norovirus in food because of its high sensitivity and specificity, which can effectively prevent the harm to human body caused by Norovirus in food. Therefore, the enrichment of Norovirus, the extraction of nucleic acid and the detection of Noroviruses by RT-PCR were reviewed in order to lay a foundation for controlling the harm of Norovirus to human body.
【作者单位】： 宜春职业技术学院医学基础部;
【分类号】：R155.5
本文编号：2330596
[Abstract]:Norovirus is a goblet virus that infects the body through food and causes acute gastroenteritis. Norovirus is more and more widespread, causing more and more harm. However, it is difficult to detect the presence of Norovirus in food precisely because of the difficulty of enrichment and nucleic acid extraction of Norovirus and the lack of standard and effective methods for detection of Norovirus. At present, fluorescent quantitative PCR is widely used in the detection of Norovirus in food because of its high sensitivity and specificity, which can effectively prevent the harm to human body caused by Norovirus in food. Therefore, the enrichment of Norovirus, the extraction of nucleic acid and the detection of Noroviruses by RT-PCR were reviewed in order to lay a foundation for controlling the harm of Norovirus to human body.
【作者单位】： 宜春职业技术学院医学基础部;
【分类号】：R155.5
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