脱氢表雄酮对大鼠脂肪代谢的影响及机理

发布时间：2018-11-19 13:29

【摘要】：目的:以雄性Sprague-Dawley(SD)大鼠为对象,探讨日粮中添加不同剂量的脱氢表雄酮(dehydroepiandrosterone,DHEA)对大鼠脂肪代谢常规生化指标及相关基因表达的影响。方法:将60只雄性SD大鼠随机分为对照组,DHEA低、中、高剂量组,各处理组大鼠分别灌胃0、25、50、100 mg/(kg·d)的DHEA(以体质量计,下同),连续灌胃8周,测定大鼠血脂、肝脂水平及肝脏脂肪代谢相关基因表达量。结果:DHEA中剂量组大鼠体质量显著低于对照组(P0.05)。中剂量的DHEA可极显著降低大鼠血清中甘油三酯和血糖含量(P0.01),低、中剂量的DHEA可显著升高大鼠血清高密度脂蛋白胆固醇含量(P0.05)。脂肪代谢合成相关基因分析结果表明,低、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中脂肪酸合成酶(fatty acid synthase,FAS)m RNA表达水平(P0.05或P0.01);与对照组相比,不同剂量的DHEA均可显著或极显著降低大鼠肝脏组织中固醇调节元件结合蛋白-1(sterol regulatory element binding protein-1,SREBP-1)m RNA和肝细胞核因子-4(hepatocyte nuclear factor-4,HNF-4)m RNA表达水平(P0.05或P0.01),但对乙酰辅酶A羧化酶(acetyl coenzyme A carboxylase,ACC)m RNA表达水平均无显著影响(P0.05)。脂肪代谢分解相关基因分析结果表明,不同剂量DHEA均可显著或极显著降低大鼠肝脏组织中酰基辅酶A氧化酶(acyl coenzyme A oxidase,ACO)m RNA表达水平(P0.05或P0.01);与对照组相比,低、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中肉毒碱棕榈酰转移酶-1(carnitine palmitoyl transterase-1,LCPT-1)m RNA和过氧化物酶体增殖物激活受体α(peroxisome proliferator activated receptor-α,PPARα)m RNA表达水平(P0.05或P0.01);中、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中脂肪三酰甘油脂肪酶(adipose triglyceride lipase,ATGL)m RNA表达水平(P0.05或P0.01)。结论:长期灌胃DHEA可影响雄性大鼠脂肪代谢途径中关键因子基因的表达,最终降低大鼠体内脂肪的沉积。
[Abstract]:Objective: to investigate the effects of different doses of Dehydroepiandrosterone (dehydroepiandrosterone,DHEA) on the routine biochemical indexes of fat metabolism and the expression of related genes in male Sprague-Dawley (SD) rats. Methods: sixty male SD rats were randomly divided into control group, low, medium and high dose DHEA groups. Rats in each treatment group were fed with DHEA (by body mass meter, the same below) for 250 mg/ (kg d), respectively, and blood lipid was measured for 8 weeks. The level of liver fat and the expression of genes related to hepatic fat metabolism. Results: the body mass of DHEA group was significantly lower than that of control group (P 0.05). Middle dose of DHEA could significantly reduce serum triglyceride and blood glucose content (P0.01), and middle dose of DHEA could significantly increase the content of serum HDL cholesterol (P0.05). The results of gene analysis showed that low and high dose of DHEA could significantly or extremely reduce the level of (fatty acid synthase,FAS) m RNA expression of fatty acid synthase in rat liver (P0.05 or P0.01). Compared with the control group, different doses of DHEA significantly or extremely decreased steroid regulatory element binding protein-1 (sterol regulatory element binding protein-1,SREBP-1) m RNA and hepatic nuclear factor-4 (hepatocyte nuclear factor-4, in rat liver tissue. HNF-4) m RNA expression level (P0.05 or P0.01), but no significant effect on acetyl-CoA carboxylase (acetyl coenzyme A carboxylase,ACC) m RNA expression (P0.05). The results of adipose catabolism related gene analysis showed that different doses of DHEA could significantly or extremely reduce the expression level of acyl coenzyme A oxidase (acyl coenzyme A oxidase,ACO) m RNA in rat liver (P0.05 or P0.01). Compared with the control group, high dose of DHEA could significantly or extremely reduce carnitine palmitoyltransferase-1 (carnitine palmitoyl transterase-1,LCPT-1) m RNA and peroxisome proliferator-activated receptor 伪 (peroxisome proliferator activated receptor- 伪. The expression level of PPAR 伪) m RNA (P0.05 or P0.01); High dose of DHEA could significantly or extremely reduce the level of (adipose triglyceride lipase,ATGL) m RNA expression of fatty triglyceride lipase in rat liver (P0.05 or P0.01). Conclusion: Long-term oral administration of DHEA can affect the expression of key factor genes in fat metabolism pathway of male rats and ultimately reduce fat deposition in rats.
【作者单位】：南京农业大学农业部动物生理生化重点实验室;
【分类号】：R151

【参考文献】