青春期前氯吡脲暴露对雌性大鼠生殖发育的影响

发布时间：2018-12-08 06:57

【摘要】：目的探讨青春期前氯吡脲暴露对雌性大鼠生殖发育的影响。方法将68只初断乳(出生后21 d)的SPF级SD雌性大鼠按体重随机分为4组,分别为对照(植物油)组和0.06、6、600 mg/kg氯吡脲染毒组,每组17只。采用经口灌胃方式进行染毒,连续染毒21 d。检测大鼠阴道开口时间、动情周期、血清生化指标、促甲状腺激素(TSH)、甲状腺激素(T4)及组织病理学的变化。结果与对照组比较,仅600 mg/kg氯吡脲染毒组大鼠的阴道开口时间均延迟,血清总蛋白(TP)、白蛋白(ALB)、肌酐(CREA)水平均较低,谷氨酸氨基转移酶(ALT)水平均较高;0.06、6 mg/kg氯吡脲染毒组大鼠血清丙氨酸氨基转移酶(AST)水平较高,差异有统计学意义(P0.05);血清TSH、T4、碱性磷酸酶(ALP)、尿素氮(BUN)的水平均无明显变化。子宫和卵巢组织学观察表现出性周期延迟或紊乱。结论高剂量氯吡脲可能诱导雌性大鼠青春期启动延迟,影响其生长发育。
[Abstract]:Objective to investigate the effect of prepubertal clopidazone exposure on reproductive development in female rats. Methods Sixty-eight SPF grade SD female rats were randomly divided into 4 groups according to their body weight: control group (vegetable oil) group and 0.06 ~ 6600 mg/kg clopiramide group with 17 rats in each group. Oral administration was used for 21 days. The changes of vaginal opening time, estrus cycle, serum biochemical indexes, thyroid hormone (TSH), (T4) and histopathology were measured. Results compared with the control group, the vaginal opening time was delayed, the serum total protein (TP), albumin (ALB), creatinine (CREA) level was lower, and the glutamate aminotransferase (ALT) level was higher in the 600 mg/kg clopidazone group than in the control group. The level of serum alanine aminotransferase (AST) was higher in 0.06 mg/kg clopicarbazone group (P0.05), while the serum TSH,T4, alkaline phosphatase (ALP), urea nitrogen (BUN) level did not change significantly. Histological observations of the uterus and ovary showed delayed or disordered sexual cycle. Conclusion High dose clopicarbazone may induce puberty onset delay and affect the growth and development of female rats.
【作者单位】：四川大学轻纺与食品学院;四川农业大学无公害农药研究实验室;四川大学华西公共卫生学院;
【分类号】：R114

【参考文献】