硫化氢调节硫代乙酰胺的肝肾损伤

发布时间：2019-02-12 22:56

【摘要】：目的观察硫化氢对硫代乙酰胺(thioacetamide,TAA)致肝肾功能损伤的影响。方法雄性SD大鼠24只,随机分为:TAA诱导肝损伤组、正常对照组、TAA+硫氢化钠组和TAA+炔丙基甘氨酸组,每组6只。用6%的TAA按照首日600 mg/kg对TAA诱导肝损伤组及TAA+硫氢化钠组和TAA+炔丙基甘氨酸组腹腔注射,24 h后采用300 mg/kg腹腔注射。首日于TAA注射之前1 h腹腔注射硫氢化钠(0.15 mmol/kg)和炔丙基甘氨酸(30 mg/kg),24 h腹腔注射剂量减半,48 h处死动物,测定血清中的硫化氢、肝肾功能以及肝肾病理学变化。所有动物自由饮食,收集用药后24 h尿量。结果给予TAA后48 h肝病理损伤严重,硫氢化钠和炔丙基甘氨酸分别加重和减轻肝损害。TAA可致ALT升高为(980.0±32.0)U/L,加用硫氢化钠ALT增加为(1095.6±684.2)U/L,而炔丙基甘氨酸使ALT恢复到(66.3±8.32)U/L。TAA使动物肾功能明显受损,即BUN:(11.62±6.0)vs(6.86±0.8)mmol/L(P0.05);UA:(198±56.0)vs(145.3±23.76)μmol/L(P0.05);Cr:(42.8±4.4)vs(34.72±2.12)μmol/L(P0.05);硫氢化钠明显减轻TAA的肾功能损伤,BUN:(8.4±1.9)vs(11.62±6.0)mmol/L,Cr:(32.6±8.2)vs(42.8±4.4)μmol/L(P0.05);炔丙基甘氨酸则加重TAA肾损伤,Cr:(56.7±14.9)vs(30.8±4.4)μmol/L(P0.05)。在TAA组动物24 h尿量较正常组明显减少,(9.2±2.5)vs(20.5±6.7)ml(P0.01);硫氢化钠使之轻度恢复,(11.7±1.5)vs(9.2±2.5)ml(P0.05);在炔丙基甘氨酸干预后,尿量进一步减少,(4.2±1.3)vs(9.2±2.5)ml(P0.01)。结论硫化氢在加重肝损害的同时改善肾功能的作用。
[Abstract]:Objective to observe the effect of hydrogen sulfide on hepatic and renal function injury induced by thioacetamide (thioacetamide,TAA). Methods Twenty-four male SD rats were randomly divided into TAA induced liver injury group, normal control group, TAA sodium sulfide group and TAA propylglycine group with 6 rats in each group. 6% TAA was used to induce liver injury induced by TAA and intraperitoneal injection of TAA sodium sulphide and TAA propargyl glycine at the first day of 600 mg/kg. After 24 hours, 300 mg/kg was injected intraperitoneally. Sodium sulfide (mmol/kg) and propargyl glycine (30 mg/kg) were injected intraperitoneally 1 hour before TAA injection on the first day, and the dose was halved at 24 h. The animals were killed at 48 h, and the serum hydrogen sulfide was determined. Liver and kidney function and pathological changes of liver and kidney. All animals were fed freely and the urine volume was collected 24 hours after medication. Results liver pathological damage was severe 48 hours after TAA, and liver damage was aggravated and alleviated by sodium sulfohydride and propargyl glycine respectively. ALT was increased to (980.0 卤32.0) U / L by TAA and ALT was increased to (1095.6 卤684.2) U / L by adding sodium sulphide. However, propargyl glycine restored ALT to (66.3 卤8.32) U/L.TAA and significantly damaged the renal function, that is, BUN: (11.62 卤6.0) vs (, 6.86 卤0.8) mmol/L (P0.05). UA: (198 卤56.0) vs (145.3 卤23.76) 渭 mol/L (P0.05); Cr: (42.8 卤4.4) vs (34.72 卤2.12) 渭 mol/L (P0.05); Sodium sulfide significantly alleviated the renal function injury in TAA patients (, BUN: (8.4 卤1.9) vs (11.62 卤6.0) mmol/L,Cr: (32.6 卤8.2) vs (42.8 卤4.4) 渭 mol/L (P0.05). Propargyl glycine aggravated TAA renal injury, Cr: (56.7 卤14.9) vs (30.8 卤4.4 渭 mol/L (P0.05). The urine volume in TAA group was significantly lower than that in normal group (9.2 卤2.5) vs (20.5 卤6.7) ml (P0.01), sodium bisulfide slightly recovered (11.7 卤1.5) vs (9.2 卤2.5) ml (P 0.05). After intervention with propargyl glycine, urine volume was further decreased (4.2 卤1.3) vs (9.2 卤2.5) ml (P 0.01). Conclusion hydrogen sulfide can not only aggravate liver damage but also improve renal function.
【作者单位】：无锡市第五人民医院脂肪肝科;中国医科大学附属第一医院消化科;杭州市西溪医院内科;浙江省人民医院传染科;
【基金】：中国肝炎基金会天晴肝病基金资助项目(20120070) 杭州科委医疗卫生及重点专科专病科研攻关专项(20120633B06)
【分类号】：R114

【参考文献】