新疆喀什地区母乳中乳酸菌的多样性分析
发布时间：2019-06-10 18:49
【摘要】：【目的】对新疆喀什地区母乳中乳酸菌多样性进行分析。【方法】采用菌落培养、显微镜观察、Repetitive genomic fingerprinting(Rep-PCR)指纹图谱和16S r RNA基因序列分析相结合的方法研究母乳中乳酸菌的菌群分布。【结果】从11份母乳中共分离出乳酸菌193株,利用16S r RNA基因序列同源分析和系统发育树对代表菌株进行了分子鉴定,193株乳酸菌隶属于4个属,分别为Lactobacillus(22株)、Streptococcus(42株)、Lactococcus(40株)、Enterococcus(89株)。其中,Enterococcus所占比例最大,达到46%。【结论】新疆喀什地区母乳中乳酸菌多样性丰富,极具开发潜力,将为开发母乳中的益生菌以及安全可靠的微生态制剂提供理论依据。
[Abstract]:[objective] to analyze the diversity of lactic acid bacteria in breast milk in Kashi area of Xinjiang. [methods] colony culture and microscope were used to observe the diversity of lactic acid bacteria in breast milk. Repetitive genomic fingerprinting (Rep-PCR) fingerprinting and 16s r RNA gene sequence analysis were used to study the distribution of lactic acid bacteria in breast milk. [results] 193 strains of lactic acid bacteria were isolated from 11 samples of breast milk. The representative strains were identified by 16s r RNA gene sequence homology analysis and phylogenetic tree. 193 strains of lactic acid bacteria belonged to 4 genus, which were Lactobacillus (22 strains of), Streptococcus (, 42 strains of), Lactococcus (, 40 strains of), Enterococcus (89 strains). Among them, Enterococcus accounted for the largest proportion, reaching 46%. [conclusion] Lactic acid bacteria in breast milk in Kashi area of Xinjiang are rich in diversity and have great potential for development, which will provide theoretical basis for the development of probiotics and safe and reliable microecological agents in breast milk.
【作者单位】： 新疆石河子大学食品学院;
【基金】：新疆兵团现代农业科技攻关与成果转化项目(No.2015AC003)~~
【分类号】：R151
，
本文编号：2496658
[Abstract]:[objective] to analyze the diversity of lactic acid bacteria in breast milk in Kashi area of Xinjiang. [methods] colony culture and microscope were used to observe the diversity of lactic acid bacteria in breast milk. Repetitive genomic fingerprinting (Rep-PCR) fingerprinting and 16s r RNA gene sequence analysis were used to study the distribution of lactic acid bacteria in breast milk. [results] 193 strains of lactic acid bacteria were isolated from 11 samples of breast milk. The representative strains were identified by 16s r RNA gene sequence homology analysis and phylogenetic tree. 193 strains of lactic acid bacteria belonged to 4 genus, which were Lactobacillus (22 strains of), Streptococcus (, 42 strains of), Lactococcus (, 40 strains of), Enterococcus (89 strains). Among them, Enterococcus accounted for the largest proportion, reaching 46%. [conclusion] Lactic acid bacteria in breast milk in Kashi area of Xinjiang are rich in diversity and have great potential for development, which will provide theoretical basis for the development of probiotics and safe and reliable microecological agents in breast milk.
【作者单位】： 新疆石河子大学食品学院;
【基金】：新疆兵团现代农业科技攻关与成果转化项目(No.2015AC003)~~
【分类号】：R151
，
本文编号：2496658
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