马尾松花粉提取物对前列腺肥大小鼠的影响
发布时间：2019-06-19 03:38
【摘要】：前列腺肥大(BPH)是老年男性的常见疾病，为患者的身体和心灵造成了很大的损害。目前治疗BPH的主流药物为5α-还原酶抑制剂和α1-肾上腺素受体阻滞剂，但它们均存在一定的副作用。马尾松花粉提取物(MPPE)是一种具有良好的抗炎和抗氧化活性的天然植物提取物，其营养丰富，无毒副作用。很多研究表明，MPPE可用于改善BPH。本实验拟采用腹腔注射丙酸睾酮（5mg/kg·d）的方法建立小鼠BPH模型，通过探究MPPE以及SQ、PGY两种复方对小鼠BPH的影响，为松花粉进一步开发为抗BPH药物或保健品提供理论依据。实验包括以下几部分： 第一，，MPPE对小鼠BPH的预防作用探究：实验周期为6周，前两周三个松花粉组小鼠预先灌胃相应剂量的MPPE。后四周，在腹腔注射丙酸睾酮建立BPH模型的同时给予三种不同剂量（20、40、80mg/kg）的MPPE。实验结束时收集小鼠尿液，进行代谢组学分析，然后处死小鼠收集血清测定相关生理指标，剖取前列腺制作石蜡切片，观察组织形态变化。 结果显示，与空白组相比，模型组小鼠的前列腺重量、前列腺指数显著增加，前列腺上皮细胞增生，向腺腔内折叠呈绒毛状，形态结构具有明显的BPH特征。三种剂量的MPPE作用效果有一定差异。与模型组相比，20mg/kg MPPE保护作用较小，而40、80mg/kgMPPE组的前列腺指数、炎性细胞因子含量、DHT浓度及PACP、NOS的活性均有明显的降低，NO的生成也相应减少。其中，80mg/kg的MPPE效果最好。PLS-DA结果与生化指标基本一致，对照组与模型组小鼠完全分开，说明BPH小鼠的尿液代谢物发生了明显的变化。非那雄胺组最接近空白组，MPPE的保护作用比较明显，但并未使小鼠完全恢复健康。 第二，MPPE对小鼠BPH的治疗作用探究：与预防作用部分实验不同的是，治疗作用实验先腹腔注射丙酸睾酮4周建立BPH模型。然后再分别灌胃不同浓度的MPPE治疗4周。结果显示，模型组小鼠的前列腺重量、前列腺指数显著增加，前列腺组织形态具有明显的BPH特征。20mg/kg MPPE作用较小，80mg/kg的MPPE效果最好。BPH小鼠的尿液代谢物发生了明显的变化，MPPE作用效果比较明显，但并未使小鼠完全恢复健康。非那雄胺对BPH的改善效果最佳。 另外，对PI百分抑制率进行对比，预防试验中三个剂量MPPE的抑制率分别为26.09%、52.17%、69.57%，而治疗实验的则分别为33.33%、57.14%、76.19%，可以看出MPPE对BPH的预防作用优于其治疗作用。 第三，MPPE及SQ、PGY复方对小鼠BPH的影响：实验具体步骤与MPPE治疗作用实验一致，1-4周：建模；5-8周：治疗。实验结束后测定相关指标。实验结果显示，SQ、PGY两个复方的抗BPH活性明显优于MPPE。且SQ的效果比PGY稍好，但两者差异没有统计学意义。 以上结果表明，（1）MPPE对BPH的预防作用优于其治疗作用，推测可能的原因是因为一边建模一边给药，MPPE及时的发挥了保护作用，减少了小鼠患BPH的可能；（2）SQ、PGY复方的效果优于MPPE，且SQ的效果比PGY稍好。说明SQ、PGY两种中草药与MPPE复合后可以起到一定的强化作用，能更好的改善BPH病症，对机体产生显著的保护作用；（3）MPPE可以降低BPH病理症状及相关生化指标，调节标志性代谢物的水平，且具有一定的剂量依赖性，MPPE的抗BPH作用机理可能与其抗炎抗氧化活性有关。
[Abstract]:Prostatic hypertrophy (BPH) is a common disease in the elderly, causing great damage to the body and mind of the patient. Currently, the main drug for treating BPH is a 5-dose reductase inhibitor and a 1-epinephrine receptor blocker, but all of them have certain side effects. Pinus massoniana pollen extract (MPPE) is a natural plant extract with good anti-inflammatory and antioxidant activity, and has rich nutrition and no toxic and side effects. Many studies have shown that MPPE can be used to improve BPH. In this experiment, a mouse BPH model was established by means of intraperitoneal injection of testosterone propionate (5 mg/ kg 路 d), and the effect of two compounds of MPPE and SQ and PGY on the BPH of mice was investigated. The experiment includes the following parts: First, the effect of MPPE on the prevention of BPH in mice: the experimental period is 6 weeks, and the corresponding dose of MPP in the three pine pollen group in the first two weeks in advance E. Three different doses of MPP (20,40,80 mg/ kg) were administered at the same time of the intraperitoneal injection of testosterone propionate to establish a BPH model and E. collecting the urine of the mouse at the end of the experiment, performing metabonomics analysis, then executing the mouse to collect the relevant physiological indexes of the serum, cutting the prostate to make the paraffin section, and observing the morphological change of the tissue; The results showed that, compared with the blank group, the prostate weight and the prostate index of the model group mice were significantly increased, the prostatic epithelial cells were hyperplastic, folded in the glandular cavity, and the morphological structure had obvious BP. H. The effect of three doses of MPPE is one. Compared with the model group, the protective effect of 20 mg/ kg of MPPE is small, and the prostate index, the inflammatory cytokine content, the DHT concentration and the PACP and the NOS activity of the 40 and 80 mg/ kg MPPE group are obviously reduced, and the generation of NO also The MPPE effect of 80 mg/ kg should be reduced. The results showed that the results of PLS-DA were basically consistent with the biochemical indexes, and the control group was completely separated from the model group mice, indicating that the urine metabolites in BPH mice were obviously The protective effect of MPPE was more obvious than that in the blank group, but it did not completely change the mice. Complex health. Second, the effect of MPPE on the treatment of BPH in mice: It is different from the part of the preventive action: the treatment effect is first established by intraperitoneal injection of testosterone propionate for 4 weeks. BPH model. Then, the different concentrations of MPP were given. E-treatment for 4 weeks. The results showed that the prostate weight and the prostate index of the model group were significantly increased, and the prostate tissue morphology had obvious BPH characteristics. The MPPE of 20 mg/ kg was less and the MPP of 80 mg/ kg was less. E effect is the best. The urine metabolites in BPH mice have changed significantly, and the effect of MPPE is more obvious, but the mice are not finished. All-in-health. The change of the non-androgenic amine to the BPH In addition, the inhibition rate of three doses of MPPE in the test was 26.09%, 52.17% and 69.57%, respectively, and 33.33%, 57.14% and 76.19%, respectively, and the preventive effect of MPPE on BPH can be seen. The effect of the combination of the third, the MPPE and the SQ and the PGY on the BPH of the mice: the experimental specific steps are consistent with the experimental results of the treatment of the MPPE, and 1-4 weeks: construction Module;5-8 weeks: Treatment. Experiment The correlation index was determined after the end of the experiment. The results showed that the two compounds of SQ and PGY were anti-BPH. The performance of SQ is better than MPPE, and the effect of SQ is slightly better than that of PGY, but both The results show that (1) MPPE is superior to the therapeutic effect of MPPE in the prevention of BPH, and the possible cause is that MPPE plays a protective role in a timely manner, which reduces the possibility of the development of BPH in the mice. (2) The effect of SQ and PGY is superior to that of MPPE. the effect of the SQ is slightly better than that of the PGY, and the combination of the two Chinese herbal medicines and the MPPE of the SQ and PGY can play a certain strengthening effect, can better improve the BPH condition, and has a remarkable protective effect on the body; and (3) the MPPE can reduce the pathological symptoms of the BPH and the related biochemical indexes, regulating the level of the symbolic metabolite and has a certain dose-dependence, and the mechanism of the anti-BPH action of the MPPE may
【学位授予单位】：山东师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R151
本文编号：2502048
[Abstract]:Prostatic hypertrophy (BPH) is a common disease in the elderly, causing great damage to the body and mind of the patient. Currently, the main drug for treating BPH is a 5-dose reductase inhibitor and a 1-epinephrine receptor blocker, but all of them have certain side effects. Pinus massoniana pollen extract (MPPE) is a natural plant extract with good anti-inflammatory and antioxidant activity, and has rich nutrition and no toxic and side effects. Many studies have shown that MPPE can be used to improve BPH. In this experiment, a mouse BPH model was established by means of intraperitoneal injection of testosterone propionate (5 mg/ kg 路 d), and the effect of two compounds of MPPE and SQ and PGY on the BPH of mice was investigated. The experiment includes the following parts: First, the effect of MPPE on the prevention of BPH in mice: the experimental period is 6 weeks, and the corresponding dose of MPP in the three pine pollen group in the first two weeks in advance E. Three different doses of MPP (20,40,80 mg/ kg) were administered at the same time of the intraperitoneal injection of testosterone propionate to establish a BPH model and E. collecting the urine of the mouse at the end of the experiment, performing metabonomics analysis, then executing the mouse to collect the relevant physiological indexes of the serum, cutting the prostate to make the paraffin section, and observing the morphological change of the tissue; The results showed that, compared with the blank group, the prostate weight and the prostate index of the model group mice were significantly increased, the prostatic epithelial cells were hyperplastic, folded in the glandular cavity, and the morphological structure had obvious BP. H. The effect of three doses of MPPE is one. Compared with the model group, the protective effect of 20 mg/ kg of MPPE is small, and the prostate index, the inflammatory cytokine content, the DHT concentration and the PACP and the NOS activity of the 40 and 80 mg/ kg MPPE group are obviously reduced, and the generation of NO also The MPPE effect of 80 mg/ kg should be reduced. The results showed that the results of PLS-DA were basically consistent with the biochemical indexes, and the control group was completely separated from the model group mice, indicating that the urine metabolites in BPH mice were obviously The protective effect of MPPE was more obvious than that in the blank group, but it did not completely change the mice. Complex health. Second, the effect of MPPE on the treatment of BPH in mice: It is different from the part of the preventive action: the treatment effect is first established by intraperitoneal injection of testosterone propionate for 4 weeks. BPH model. Then, the different concentrations of MPP were given. E-treatment for 4 weeks. The results showed that the prostate weight and the prostate index of the model group were significantly increased, and the prostate tissue morphology had obvious BPH characteristics. The MPPE of 20 mg/ kg was less and the MPP of 80 mg/ kg was less. E effect is the best. The urine metabolites in BPH mice have changed significantly, and the effect of MPPE is more obvious, but the mice are not finished. All-in-health. The change of the non-androgenic amine to the BPH In addition, the inhibition rate of three doses of MPPE in the test was 26.09%, 52.17% and 69.57%, respectively, and 33.33%, 57.14% and 76.19%, respectively, and the preventive effect of MPPE on BPH can be seen. The effect of the combination of the third, the MPPE and the SQ and the PGY on the BPH of the mice: the experimental specific steps are consistent with the experimental results of the treatment of the MPPE, and 1-4 weeks: construction Module;5-8 weeks: Treatment. Experiment The correlation index was determined after the end of the experiment. The results showed that the two compounds of SQ and PGY were anti-BPH. The performance of SQ is better than MPPE, and the effect of SQ is slightly better than that of PGY, but both The results show that (1) MPPE is superior to the therapeutic effect of MPPE in the prevention of BPH, and the possible cause is that MPPE plays a protective role in a timely manner, which reduces the possibility of the development of BPH in the mice. (2) The effect of SQ and PGY is superior to that of MPPE. the effect of the SQ is slightly better than that of the PGY, and the combination of the two Chinese herbal medicines and the MPPE of the SQ and PGY can play a certain strengthening effect, can better improve the BPH condition, and has a remarkable protective effect on the body; and (3) the MPPE can reduce the pathological symptoms of the BPH and the related biochemical indexes, regulating the level of the symbolic metabolite and has a certain dose-dependence, and the mechanism of the anti-BPH action of the MPPE may
【学位授予单位】：山东师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R151
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