负载阿维菌素壳聚糖胶囊的制备及性能研究

发布时间：2018-06-16 03:54

本文选题：多糖 + 纳米颗粒　；参考：《大连海事大学》2017年硕士论文

【摘要】：随着我国社会经济的发展,各地高速公路建设飞速发展。但是,高速公路的建设也不同程度地影响和破坏了生态环境,高速公路绿化是可持续发展战略的具体内容,承担着保护和恢复高速公路周边自然生态环境的责任,为了充分发挥绿色植被的功能,高速公路绿化带的治理更要降低对环境的污染。制备具有缓释效果的纳米农药以提高农药的利用率,减轻对环境的污染和对人类的危害,显得尤为重要。天然多糖由于其具有高度稳定、安全、无毒、可生物降解的特点,在药物递送系统领域中受到越来越多的关注,已成为制备纳米载体的最理想的材料。本文的课题实验主要包括两部分。第一部分为载体的制备及性能研究。选取壳聚糖与海藻酸钠两种多糖材料为原料,通过静电自组装的方法制备了载药的纳米胶囊。利用Nano-Size、傅里叶红外变化光谱(FTIR)、扫描电镜等方法对得到的纳米颗粒进行表征,并测定了其抑菌性能。通过响应面分析方法,研究了影响纳米颗粒制备的关键影响因素,成功制备了粒径可控,溶液均一的纳米颗粒;抑菌试验表明壳聚糖纳米颗粒具有良好的抑菌效果。第二部分为载药微胶囊的制备及性能研究。选用了具有光谱杀菌效果的阿维菌素作为药物模型,选用具有天然安全的卵磷脂作为表面活性剂,通过改变壳聚糖浓度及药物的浓度,添加表面活性剂等方法,研究了制备条件对载药胶囊包封率及缓释效果的影响。通过高效液相色谱法以及紫外分光光度计的方法,建立了阿维菌素0.1μg/L-100mg/L的检测方法,可以准确方便的检测阿维菌素的浓度;通过傅里叶变换光谱(FTIR),Nano-Size,显微镜等对载药胶囊的表面形态、粒径及分布等进行了表征,制备得到了粒径在2μm左右、分布均匀的载药胶囊,药物包封率高达93%;与原药相比,该制剂阿维菌素释放速度显著降低,72小时内累积释放量仅为41%,实现了药物的缓释效果,达到了实验预期目标。
[Abstract]:With the development of our social economy, highway construction is developing rapidly. However, the construction of freeway also affects and destroys the ecological environment to some extent, the highway greening is the concrete content of the sustainable development strategy, and bears the responsibility of protecting and restoring the natural ecological environment around the expressway. In order to give full play to the function of green vegetation, the treatment of highway green belt should reduce the pollution to the environment. It is very important to prepare nanometer pesticides with slow release effect to improve the utilization rate of pesticides, reduce environmental pollution and harm to human beings. Due to its high stability, safety, non-toxicity and biodegradability, natural polysaccharides have attracted more and more attention in the field of drug delivery systems, and have become the most ideal materials for the preparation of nano-carriers. The experiment of this paper mainly includes two parts. The first part is the preparation and performance study of the carrier. Chitosan and sodium alginate were used as raw materials to prepare drug-loaded nano-capsules by electrostatic self-assembly. The nanoparticles were characterized by Nano-Size, FTIR and SEM, and their bacteriostatic properties were determined. The key factors affecting the preparation of nanoparticles were studied by response surface analysis, and the nanoparticles with controllable particle size and uniform solution were successfully prepared, and the bacteriostatic test showed that chitosan nanoparticles had good bacteriostatic effect. The second part is about the preparation and properties of drug-loaded microcapsules. Abamectin with spectral bactericidal effect was selected as drug model, and lecithin with natural safety was selected as surfactant. By changing the concentration of chitosan and drug, adding surfactant, etc. The effects of preparation conditions on encapsulation efficiency and sustained release efficiency of drug-loaded capsules were studied. By means of high performance liquid chromatography and ultraviolet spectrophotometer, a method for the detection of avermectin 0.1 渭 g / L-100 mg / L was established, which can be used to detect the concentration of avermectin accurately and conveniently. The surface morphology, particle size and distribution of drug-loaded capsules were characterized by Fourier transform spectroscopy (FTIR), Nano-Sizeand microscope. The encapsulation efficiency of drug-loaded capsules with a particle size of about 2 渭 m and uniform distribution was as high as 93%. The release rate of abamectin significantly decreased the cumulative release of abamectin within 72 hours was only 41. The drug release effect was achieved and the expected goal of the experiment was achieved.
【学位授予单位】：大连海事大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：U418.9

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