聚磷微生物的筛选及其对富营养化水体中磷的去除效果研究
发布时间：2019-06-03 23:37
【摘要】：近年来,湖泊水域出现水体富营养化现象。磷是水体富营养的限制性因子,因此磷的去除是富营养化水体防治的关键。与吸附法除磷、化学沉淀法除磷相比,生物除磷法是目前应用前景较好的除磷技术,成为目前的研究热点。生物除磷的关键是除磷微生物。因此,筛选除磷微生物并掌握其生长特性对提高生物除磷效果具有重大的意义和较大的实用价值。本论文旨在为巢湖关镇河的水域污染治理提供一定的理论依据。研究内容主要包括除磷微生物的筛选、纯化、种类鉴定、生长性能、除磷效果以及自然水体应用研究。从长期受污染的湖泊底泥中分离、纯化筛选出两株聚磷菌,分别为菌株33和菌株35。经生理生化及16SrDNA鉴定,菌株33是丁香假单胞菌,假单胞菌属；菌株35是假单胞菌,假单胞菌属。在培养基中做好氧纯培养,结果表明,菌株33的最佳培养时间为24h,pH值为5-9；菌株35的最佳培养时间为24h,pH值为5-8。本文采用正交实验法研究总磷(TP)浓度、pH值、温度对聚磷菌总磷去除效果的研究。结果表明,菌株33的最佳除磷条件为TP浓度为2.0mg/l,pH值为9.0,温度为15℃,菌株33的除磷率为64.68%；菌株35的最佳除磷条件为TP浓度为2.0mg/l,pH值为5.0,温度为15℃,菌株35的除磷率为80.43%。为了研究菌株除磷中磷的去除分布,分析了菌株的胞内和胞外磷含量。菌株33的胞内TP含量占生物除磷总量的82.15%,EPS中TP含量占生物除磷总量的3.69%；菌株35胞内TP含量占生物除磷总量的72.12%,EPS中TP含量占生物除磷总量的5.53%。考察筛选得到的菌株33和菌株35在自然水体的应用,结果表明菌株33、菌株35在自然水体中除磷率不高且除磷效果不稳定,这可能与菌株在自然水体中生长繁殖和生理活性有关,需要进一步研究其在自然水体中的适用性。
[Abstract]:In recent years, eutrophication occurs in lake waters. Phosphorus is the limiting factor of eutrophication, so the removal of phosphorus is the key to the prevention and control of eutrophication. Compared with adsorption and chemical precipitation, biological phosphorus removal is a promising phosphorus removal technology, and has become a hot research topic at present. The key to biological phosphorus removal is phosphorus removal microorganisms. Therefore, it is of great significance and practical value to screen phosphorus removal microorganisms and master their growth characteristics in order to improve the effect of biological phosphorus removal. The purpose of this paper is to provide a theoretical basis for the water pollution control of Guanzhen River in Chaohu Lake. The research contents mainly include the screening, purification, species identification, growth performance, phosphorus removal effect and natural water application of phosphorus removal microorganisms. Two strains of phosphorus-accumulating bacteria, strain 33 and strain 35, were isolated and screened from the sediment of long-term polluted lake. By physiological, biochemical and 16SrDNA identification, strain 33 was Pseudomonas clove, Pseudomonas, and strain 35 was Pseudomonas and Pseudomonas. The results showed that the optimum culture time of strain 33 was 24 h, pH value was 5 鈮，
本文编号：2492308
[Abstract]:In recent years, eutrophication occurs in lake waters. Phosphorus is the limiting factor of eutrophication, so the removal of phosphorus is the key to the prevention and control of eutrophication. Compared with adsorption and chemical precipitation, biological phosphorus removal is a promising phosphorus removal technology, and has become a hot research topic at present. The key to biological phosphorus removal is phosphorus removal microorganisms. Therefore, it is of great significance and practical value to screen phosphorus removal microorganisms and master their growth characteristics in order to improve the effect of biological phosphorus removal. The purpose of this paper is to provide a theoretical basis for the water pollution control of Guanzhen River in Chaohu Lake. The research contents mainly include the screening, purification, species identification, growth performance, phosphorus removal effect and natural water application of phosphorus removal microorganisms. Two strains of phosphorus-accumulating bacteria, strain 33 and strain 35, were isolated and screened from the sediment of long-term polluted lake. By physiological, biochemical and 16SrDNA identification, strain 33 was Pseudomonas clove, Pseudomonas, and strain 35 was Pseudomonas and Pseudomonas. The results showed that the optimum culture time of strain 33 was 24 h, pH value was 5 鈮，
本文编号：2492308
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