ApoE基因敲除小鼠海马CA3区神经元形态学和微管相关蛋白2表达变化

发布时间：2018-03-17 06:15

本文选题：载脂蛋白E基因敲除　切入点：微管相关蛋白　出处：《安徽医科大学学报》2016年11期 　论文类型：期刊论文

【摘要】：目的观察载脂蛋白E基因敲除(Apo E KO)对小鼠海马CA3区神经元形态结构改变以及微管相关蛋白2(MAP-2)表达的影响。方法 10只野生型C57BL/6J小鼠为对照组,10只Apo E基因敲除小鼠为KO组,普通饲料喂养3个月。采用分光光度计检测血脂变化;取两组小鼠脑组织,HE染色和电镜技术观察海马CA3区结构改变,免疫组织化学技术观察两组小鼠海马CA3区MAP-2表达变化,采用计算机图像分析系统检测其平均光密度(MOD)值。结果与对照组比较,KO组小鼠血浆总胆固醇、血浆三酰甘油、低密度脂蛋白含量明显升高(P0.05,P0.01)。光镜下,KO组小鼠海马CA3区锥体细胞较小,排列稀疏松散。电镜下,KO组海马神经元内线粒体肿胀变性,嵴消失;神经纤维髓鞘松懈;突触小泡数量减少,突触间隙模糊等。免疫组织化学结果显示,对照组小鼠海马神经元内MAP-2高表达,KO组含量明显减少;图像分析显示KO组CA3区MAP-2MOD值明显降低(P0.05)。结论 Apo E KO小鼠海马神经元结构出现病理改变,MAP-2表达下降,提示Apo E KO与神经退行性病变有一定联系。
[Abstract]:Objective to observe the effects of apolipoprotein E knockout (Apo E KOO) on the changes of neuronal morphology and the expression of microtubule-associated protein 2mMAP-2.Methods Ten wild-type C57BL / 6J mice were used as control group and 10 Apo E knockout mice were used as KO group. The changes of serum lipids were detected by spectrophotometer, the changes of CA3 structure in hippocampus were observed by HE staining and electron microscopy, and the changes of MAP-2 expression in hippocampal CA3 were observed by immunohistochemical technique. The average optical density (MOD) was measured by computer image analysis system. Results compared with the control group, the plasma total cholesterol and triglyceride were measured in the KO group. The content of low density lipoprotein (LDL) increased significantly in P0.05P0.010.The pyramidal cells in the hippocampal CA3 region of KO group were smaller and loosely arranged. Under electron microscope, mitochondria swelled and degenerated in hippocampal neurons, and the ridges disappeared, and the myelin sheath of nerve fibers was lax. The number of synaptic vesicles was decreased and the synaptic gap was blurred. The results of immunohistochemistry showed that the content of MAP-2 in hippocampal neurons of control group was significantly decreased. Image analysis showed that the MAP-2MOD value of CA3 in KO group was significantly lower than that of P0.05.Conclusion the expression of MAP-2 in hippocampal neurons of Apo E KO mice is decreased, suggesting that Apo EKO is associated with neurodegenerative lesions.
【作者单位】：安徽医科大学组织学与胚胎学教研室;安徽医学高等专科学校基础部;安徽医科大学综合实验室;
【基金】：安徽高校省级自然科学研究项目(编号:KJ2012A164)
【分类号】：R749.16

【相似文献】