内蒙古呼和浩特地区丙型肝炎病毒基因型分布

发布时间：2018-03-22 16:43

本文选题：丙型肝炎病毒　切入点：基因型　出处：《病毒学报》2017年01期 　论文类型：期刊论文

【摘要】：为了解内蒙古呼和浩特地区丙型肝炎病毒(HCV)基因型的分布特征,为本地区丙型肝炎的治疗、预防提供基础数据资料。收集呼和浩特地区2014年1月至2015年1月就诊的门诊和住院丙型肝炎患者血清采样标本149份,均为HCV抗体检测阳性且HCVRNA定量检测阳性。QIAGEN柱式病毒RNA提取法提取HCV RNA,反转录成cDNA,巢式PCR法扩增HCVNS5B区,对扩增片段进行测序,在NCBI BLAST上进行序列比对得到相似度最大的参考序列并确定基因型,用Megalign,Clustal W进行序列分析并建立同源关系树,分析呼和浩特地区HCV感染基因型的分布特征,以及基因型与宿主性别、年龄的关系。在测序成功的94份样本中,共检出6个基因型,1b型占73.40%(69/94),2a型占19.14%(18/94),3a、3b、6a型各占2.12%(2/94),6u型占1.06%(1/94)。性别资料明确的93例样本,男女基因型分布无显著差异(P0.05)。年龄资料明确的90例HCV样本,1+2型的患病年龄高于3+6型的患病年龄有统计学意义(P0.05)。得出内蒙古呼和浩特地区HCV感染的基因型1b为主,其次为2a型,但也有3型、6型等基因型的传入。HCV的基因型4型和5型未检测到。
[Abstract]:In order to understand the distribution characteristics of hepatitis C virus (HCV) genotype in Hohhot, Inner Mongolia, it is the treatment of hepatitis C in this area. Prevention provides basic data. 149 samples of serum samples from outpatients and inpatients with hepatitis C from January 2014 to January 2015 in Hohhot region were collected, All of them were HCV antibody positive and HCVRNA positive. QIAGEN column virus RNA extraction method was used to extract HCV RNAs, reverse transcribe into cDNAs, amplify HCVNS5B region by nested PCR method, and sequence the amplified fragments. The most similar reference sequences were obtained by sequence alignment on NCBI BLAST and genotypes were determined. The homology tree was established by using Megalignus Clustal W to analyze the distribution characteristics of HCV infection genotypes in Hohhot. And the relationship between genotype and host sex, age. Among 94 samples successfully sequenced, 6 genotypes (73.40 / 94) were found to account for 19.14% / 94 / 3b ~ (6a) and 2.12% / 94 / 94 / 6u / 1.066 / 94 / 94.93 samples with clear sex data. There was no significant difference in the distribution of male and female genotypes (P0.05A). There was a significant difference in the age of HCV type 12 (> 36) in 90 patients with age data. It was concluded that the genotype 1b of HCV infection was predominant in Hohhot, Inner Mongolia. The second genotype was 2a, but genotype 4 and genotype 5 of afferent. HCV were not detected.
【作者单位】：呼和浩特市第二医院;内蒙古医科大学;呼和浩特市结核病防治所;内蒙古第四医院;
【基金】：内蒙古自治区自然科学基金(项目号:2016MS0819);题目:荧光素酶标记的嵌合丙型肝炎病毒感染性克隆的构建
【分类号】：R512.63

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