Intron-targeted gene insertion in rice using CRISPR/Cas9: A

发布时间：2021-03-07 04:46

　　Intron-targeted gene insertion strategy using CRISPR/Cas9（clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9） has been shown to be a potential tool for crop genetic improvement by targeted mutagenesis or gene replacement of an elite allele into widely cultivated rice varieties. The rice blast resistant protein Pi-ta, differs from its susceptible counterpart, pi-ta, by a single amino acid in exon 2. To create new materials resistant to the rice blast disease, we inse... 

【文章来源】：The Crop Journal. 2020,8(03)

【文章页数】：8 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Vector construction
    2.2. Rice transformation
    2.3. Molecular characterization of the transgenic plants
3. Results and discussion
    3.1. Intron-targeted insertion of exon 2 of Pi-ta
    3.2. Genotyping of the transgenic lines
    3.3. Selection of the progeny without the transgene
    3.4. Expression analysis of the inserted Pi-ta gene
    3.5. Agronomic trait analysis and detection of potential off-target sites
    3.6. Applications in the molecular breeding
4. Conclusions
Appendix A.Supplementary data


【参考文献】：
期刊论文
[1]稻瘟病抗病基因Pita和Pib在中国水稻地方品种中的分布[J]. 杨杰,杨金欢,王军,范方军,朱金燕,曹卿,田胜尼,仲维功.  华北农学报. 2011(03)



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