玉米籽粒大小性状的QTL定位

发布时间：2021-03-18 22:11

　　籽粒大小相关性状,包括粒长、粒宽、粒厚等,对于玉米的产量与品质表现具有决定性的影响。揭示这些性状的表型特性,并发掘相应的QTL,对于玉米高产、稳产育种,以及品质改良都具有重要的实践指导意义。本研究以自育的穗位极低材料EHel（extremely low Ear Height）与测序自交系B73杂交所构建的F3分离群体为研究对象,全面鉴定了群体的粒长、粒宽、粒厚,并结合10K的SNP芯片基因型鉴定,开展了玉米极低穗位材料EHel籽粒大小性状的表型鉴定与QTL定位。主要研究结果如下:1.亲本及分离群体籽粒大小性状的表型特性表型鉴定结果显示,两个亲本EHel与B73的10-粒长（10-kernel length,KL）与10-粒宽（10-kernel width,KW）在2018与2019之间均表现出极显著差异（P<0.01）。但是EHel的10-粒厚（10-kernel thickness,KT）在两年之间差异不显著（P=0.6788）,而B73的KT在两年间则差异极显著（P=0.0098）。亲本之间的比较结果表明,在2018与2019两年间,EHel与B73的KL与KW均存在极显著... 

【文章来源】：西南大学重庆市 211工程院校 教育部直属院校

【文章页数】：68 页

【学位级别】：硕士

【文章目录】：
ABSTRACT
摘要
CHAPTER1
    1.1 GLOBAL MAIZE DISTRIBUTION,PRODUCTION,AND CONSUMPTION
        1.1.1 General overview of maize
        1.1.2 Global maize distribution
        1.1.3 Global maize production
        1.1.4 Global maize consumption
    1.2 YIELD AND KERNEL-RELATED YIELD COMPONENTS OF MAIZE
        1.2.1 Yield and kernel-related yield components of harvesting maize
        1.2.2 Documents focused on maize yield and kernels
        1.2.3 Kernel related traits and their contributions to maize yield performance
    1.3 ADVANCE IN GENETICS OF KERNEL RELATED TRAITS IN MAIZE AND OTHER CROPS
        1.3.1 Genetic dissection of kernel related traits
        1.3.2 Detection of chromosomal regions controlled kernel related traits
        1.3.3 Cloned genes for kernel related traits in maize and other crops
    1.4 MAPPING STRATEGY FOR MARKER-TRAIT ASSOCIATION
        1.4.1 QTL mapping and often-used software
        1.4.2 Association mapping
        1.4.3 Bulked segregation/sample analysis（BSA）
    1.5 HIGH-THROUGHPUT GENOTYPING VIA SNP-BASED CHIPSET
CHAPTER2 MATERIALS AND METHODS
    2.1 PLANT MATERIALS
    2.2 EXPERIMENT DESIGN AND DATA COLLECTING
    2.3 PHENOTYPIC DATA ANALYZING
    2.4 CHIPSET-BASED GENOTYPING
    2.5 SCREENING OF POLYMORPHIC SNPS AND CONSTRUCTION OF LINKAGE MAP
    2.6 QTL MAPPING FOR THREE KERNEL-SIZE RELATED TRAITS
    2.7 VERIFICATION OF CANDIDATE CHROMOSOMAL REGION VIA BULKED STRATEGY
CHAPTER3 RESULTS
    3.1 PHENOTYPIC PERFORMANCE OF THREE KERNEL-SIZE RELATED TRAITS
    3.2 CORRELATION ANALYSIS OF PHENOTYPIC TRAITS
    3.3 CONSTRUCTION OF LINKAGE MAP
    3.4 QTL MAPPING FOR THE TARGET TRAITS
    3.5 PRIMARY VERIFICATION OF TARGET CHROMOSOMAL REGION ON CHR9 VIA BULKED STRATEGY
CHAPTER4 DISCUSSION AND CONCLUSION
    4.1 DISCUSSION
    4.2 CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS



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