大豆独脚金内酯合成和信号路径相关基因全基因组分析及几个GmMAX基因功能分析

发布时间：2021-10-20 05:01

　　独脚金内酯（SLs）是一类新发现的植物激素,广泛参与植物的生长发育,并参与植物根系与土壤微生物的互作。独脚金内酯类化合物能够刺激丛枝菌根（AMF）菌丝的分枝和促进根寄生植物的种子萌发。豆科植物,如蒺藜苜蓿（Medicago truncatula）能够与根瘤菌形成植物-细菌互惠共生关系,通过根瘤这一特殊的器官进行固氮作用。独脚金内酯能够调控根系生长,茎的分枝,植物-微生物共生体的产生,同时还能够响应缺磷和各种逆境胁迫。尽管最近在模式植物中对独脚金内酯进行了大量的研究,然而大豆中独脚金内酯的生物合成及信号传导研究还很少。本研究中我们克隆了大豆独脚金内酯合成酶基因和信号传导基因。我们研究了大豆GmMAX1,GmMAX2,GmMAX3和GmMAX4,以及GmD14,GmD53,GmD27和GmPDR1等独脚金内酯的合成酶基因和信号传导基因在不同组织中的表达模式,以及在外界非生物逆境胁迫下的表达。同时我们还检测了根瘤菌感染后大豆根以及大豆根瘤不同发育阶段的相关基因的表达情况。为了进一步研究GmMAX的基因功能,我们构建了GmMAX1a,GmMAX2a,GmMAX3a和GmMAX4a四个基因的Gm... 

【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校

【文章页数】：123 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
Abbreviation
CHAPTER I Introduction
    1.1 Strigolactones(SLs):An overview
    1.2 Chemical nature of SLs
    1.3 SLs biosynthesis pathway
    1.4 Strigolactone's signaling pathway
        1.4.1 SLs perception
        1.4.2 SLs signaling
        1.4.3 SLs transports
    1.5 Biological functions of SLs
        1.5.1 SLs signaling in the control of branching
        1.5.2 SLs signaling in root development
        1.5.3 SLs and drought/salt stress
        1.5.4 Role of SLs in leaf senescence
        1.5.5 SLs elongates inter-node length
        1.5.6 Role of SLs in nodulation
    1.6 SLs are signals for plant interactions
        1.6.1 Mycorrhizal symbiosis
        1.6.2 Parasitic plants
        1.6.3 Symbiotic interaction of SLs with rhizobium spp
    1.7 Interaction with auxin
        1.7.1 Interaction with ABA
    1.8 More axillary growth(MAXs)genes and their role in SLs biosynthesis and signaling pathway
        1.8.1 Role of MAX1 in SLs biosynthesis
        1.8.2 Role of MAX2 in SLs signaling
        1.8.3 Role of MAX3(CCD7) &MAX4(CCD8)in SLs biosynthesis
    1.9 Aims of the study:The specific aims are as follow
CHAPTER II Materials and methods
    2.1 Plant materials and growth condition
    2.2 Gene cloning and vector construction
    2.3 Hairy root induction and hormone analysis:
        2.3.1 In vitro hairy root induction
        2.3.2 In-vivo hairy root induction
    2.4 GmMAXs transgenic soybean hairy root nodulation assay
    2.5 Rhizobia infection
    2.6 Generation of transgenic hairy roots or collection of nodules for RNA-seq analysis
    2.7 RNA isolation,cDNA library construction for Illumina deep sequencing
    2.8 Quantitative RT-PCR(qRT-PCR)analysis of gene expression
    2.9 Hormone extraction and analysis
    2.10 Bioinformatics analysis
    2.11 Statistical analysis
    2.12 Protein accession number
CHAPTER III Results
    3.1 Identification of SLs biosynthetic and signaling genes from soybean genome
    3.2 Tissue expression pattern of SLs biosynthesis and signaling genes
    3.3 SLs biosynthesis and signaling gene expression upon B.japonicum(USDA110)infection
    3.4 SLs biosynthesis and signaling gene expression at different stages of nodules
    3.5 Effects of GmMAXsa-knockdown on soybean nodulation
    3.6.Effect of GmMAXsa-knockdown on nodulation signaling pathway genes
    3.7 Effect of GmMAXsa knockdown on plant hormones
    3.8 Effects of GmMAX2a-knockdown on SLs biosynthesis and signaling genes
    3.9 Effect of GmMAX2a-knockdown on auxin biosynthesis genes
    3.10 GmMAX2 regulates a wide range of hormone-related genes and diverse transcription factors
    3.11 Overexpression of auxin biosynthesis gene affects the SLs biosynthesis and signaling genes
    3.12 Abiotic stresses affect the SLs biosynthesis and signaling genes
CHAPTER IV Discussion
    4.1 Soybean genome has conserved SLs biosynthesis and signaling components
    4.2 SLs are involved in soybean-rhizobia interaction and nodule development to senescence
    4.3 SLs biosynthesis is essential for nodulation in soybean
    4.4 GmMAX2-dependent SLs signaling is essential for nodulation in soybean
    4.5 SLs biosynthesis and signaling interacts with other hormone signaling
    4.6 SLs in soybean are involved in abiotic stresses
Conclusion
References
Appendices
    Appendix1
    Appendix2
    Appendix3
Acknowledgement
List of publications



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