玉米小RNA种内变异的遗传机制全基因组分析

发布时间：2022-01-27 10:54

　　长度为18-30个核苷酸（nt）的小RNA（s RNAs）广泛存在于动物、植物以及真菌中,是一类重要的RNA分子,在生物体的众多生物过程中发挥了至关重要的作用。早在上世纪80年代,人们就已经对s RNA进行研究,发现s RNAs是通过内切酶DICERLIKE（DCL）加工产生。1999年在植物中首次发现和基因转录后沉默相关的s RNAs。随后大量研究表明,s RNAs可以在转录、转录后或翻译水平上通过与靶基因序列互补配对的方式调控基因表达,从而参与植物的生长发育、代谢进程以及响应各种胁迫反应的信号通路调控,具有重要的生物学意义。s RNAs在植物中存在广泛的变异,且在同一物种内不同材料之间的变异也广泛存在,并有报道s RNAs在种内变异可能与物种内的表型变异紧密相关。然而我们对种内s RNAs变异的类型与变异的内在分子机制还不清楚,这些限制了我们对植物表型变异的理解与分子育种的实践应用。为了系统鉴定s RNAs的种内变异类型并解析其变异的分子机制,我们收集了两个玉米优良自交系B73和Mo17叶片组织的深度s RNAs测序数据,并收集了一个具有广泛遗传变异的玉米关联群体（338份优良自交... 

【文章来源】：华中农业大学湖北省211工程院校教育部直属院校

【文章页数】：97 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
List of abbreviations
1.Introduction
    1.1.Maize(Zea mays L.)is among topmost important crops worldwide
    1.2.Maize reference genome
    1.3.Small RNA(s RNA)
    1.4.Genome-wide association study(GWAS)
    1.5.Advantages of association studies
    1.6.Expression quantitative trait locus(eQTL)
    1.7.A framework of eQTL analysis
    1.8.eQTL hotspots
    1.9.Aims of the study
2.Materials and methods
    2.1.Plant Materials and growth conditions
    2.2.Software
    2.3.Library Construction and sequencing
    2.4.Small RNA data processing
        2.4.1.Quantification of s RNA sequence and s RNA species
        2.4.2.Differential expression of s RNA species
    2.5.Expression genome-wide association studies(eGWAS)analysis
    2.6.Identification and quantification of trans-eQTLs
        2.6.1.trans-e QTL hotspots identification and chi-square test
        2.6.2.Candidate gene selection
        2.6.3.Gene ontology(GO)enrichment analysis of all trans-e QTLs associated genes
3.Results
    3.1.Genetic materials and distribution of sRNAs
        3.1.1 Distribution of s RNAs and s RNA species
        3.1.2.s RNAs differential expression pattern
    3.2.Expression genome-wide association mapping(e GWAS)
    3.3.eQTL analysis of sRNA species
        3.3.1.Identification of trans-e QTL hotspots,and candidate genes
        3.3.2.GO enrichment analysis of all trans-e QTL associated genes
4.Discussion
    4.1.Various genomic features contributing to variation in sRNAs
    4.2.Genome-wide association study and eQTL mapping
    4.3.trans-eQTL hotspots and candidate genes
5.Summary and future prospects
References
Appendices
    Appendix1
    Appendix2
    Appendix3
    Appendix4
    Appendix5
    Appendix6
Acknowledgements


【参考文献】：
期刊论文
[1]AGO18b negatively regulates determinacy of spikelet meristems on the tassel central spike in maize[J]. Wei Sun,Xiaoli Xiang,Lihong Zhai,Dan Zhang,Zheng Cao,Lei Liu,Zuxin Zhang.  Journal of Integrative Plant Biology. 2018(01)



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