GraS is critical for chloroplast development and affects yie

发布时间：2022-02-22 06:21

　　Leaf color has been considered an important agronomic trait in rice（Oryza sativa L.） for a long time. The changes in leaf color affect the yield of rice. In this study, a green-revertible albino（gra S） mutant was isolated from a ⁶⁰Co-gamma-irradiated mutant pool of indica cultivar Guangzhan 63-4 S. The fine mapping indicated that gra S mutant was mapped to chromosome 1, and was located in a confined region between markers ab134 and InDel 8 with genetic distances of 0.11 and 0.06 cM, r... 

【文章来源】：Journal of Integrative Agriculture. 2020,19(11)SCICSCD

【文章页数】：13 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials,growth conditions and phenotypic characterization
    2.2. Microscopic and transmission electron microscope observations
    2.3. Extraction and determination of photosynthetic pigment
    2.4. Marker development and mapping of Gra S
    2.5. Gene cloning and sequence analysis
    2.6. Sequence and phylogenetic analysis
    2.7. Gene expression analysis
3. Results
    3.1. Morphological characteristics of gra S
    3.2. The gra S mutant produces defects in the chloroplast development
    3.3. Map-based cloning of Gra S
    3.4. Sequence analysis of Gra S
    3.5. Gra S was expressed ubiquitously and expressionpattern of genes associated with chloroplast development in graS
4. Discussion
    4.1. Gra S mutant phenotypic defects lead to reduced yields
    4.2. Gra S expression
    4.3. Gra S is involved in chloroplast development in rice
    4.4. gra S mutant can be applied in molecular breeding
5. Conclusion


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