Putrescine, spermidine, and spermine play distinct roles in
发布时间:2022-02-22 23:57
Polyamines(PAs) play diverse roles in plant growth and development, as well as responses to environmental stimuli. In this study, the effects of PAs on rice salt tolerance were investigated. Salt stress resulted in the alteration of endogenous PAs levels in rice roots and leaves, where spermidine(Spd) and spermine(Spm) contents were increased, and putrescine(Put) content was decreased. RT-qPCR analysis revealed that PAs biosynthesis-related genes ADC1, ODC, and Arginase were significantly downre...
【文章来源】:Journal of Integrative Agriculture. 2020,19(03)SCICSCD
【文章页数】:13 页
【文章目录】:
1. Introduction
2. Materials and methods
2.1. Plant materials,cultivation,and salt stress treat-ment
2.2. Extraction and analysis of PAs
2.3. RNA extraction and RT-qPCR assay
2.4. Polyamine treatments
2.5. Plant transformation
2.6. Measurement of electrolyte leakage
2.7. Quantification of chlorophyll
2.8. Determination of PAO activities
2.9. Measurement of reactive oxygen species super-oxide(O2-·)and hydrogen peroxide(H2O2)
3. Results
3.1. Impact of salt stress on polyamine levels in rice seedlings
3.2. Salt stress affects PAs biosynthesis gene expression
3.3. Exogenous Spm increases rice salt tolerance
3.4. Overexpression of SAMDC enhances rice salt tolerance
3.5. Put and Spd facilitate ROS generation in salt-stressed rice plants
3.6. Salt stress increases PAO activities in rice
4. Discussion
4.1. Polyamine accumulation improves rice salt tolerance
4.2. Rice salt tolerance may be regulated by polyam-ine homeostasis
5. Conclusion
【参考文献】:
期刊论文
[1]高温胁迫下紫花苜蓿多胺含量与耐热性的关系[J]. 邵辰光,王荟,毕玉芬. 草地学报. 2015(06)
[2]外源Spd对高温胁迫下黄瓜幼苗叶片膜脂过氧化及质子泵活性的影响[J]. 田婧,郭世荣,孙锦,王丽萍,阳燕娟,李斌. 应用生态学报. 2011(12)
本文编号:3640443
【文章来源】:Journal of Integrative Agriculture. 2020,19(03)SCICSCD
【文章页数】:13 页
【文章目录】:
1. Introduction
2. Materials and methods
2.1. Plant materials,cultivation,and salt stress treat-ment
2.2. Extraction and analysis of PAs
2.3. RNA extraction and RT-qPCR assay
2.4. Polyamine treatments
2.5. Plant transformation
2.6. Measurement of electrolyte leakage
2.7. Quantification of chlorophyll
2.8. Determination of PAO activities
2.9. Measurement of reactive oxygen species super-oxide(O2-·)and hydrogen peroxide(H2O2)
3. Results
3.1. Impact of salt stress on polyamine levels in rice seedlings
3.2. Salt stress affects PAs biosynthesis gene expression
3.3. Exogenous Spm increases rice salt tolerance
3.4. Overexpression of SAMDC enhances rice salt tolerance
3.5. Put and Spd facilitate ROS generation in salt-stressed rice plants
3.6. Salt stress increases PAO activities in rice
4. Discussion
4.1. Polyamine accumulation improves rice salt tolerance
4.2. Rice salt tolerance may be regulated by polyam-ine homeostasis
5. Conclusion
【参考文献】:
期刊论文
[1]高温胁迫下紫花苜蓿多胺含量与耐热性的关系[J]. 邵辰光,王荟,毕玉芬. 草地学报. 2015(06)
[2]外源Spd对高温胁迫下黄瓜幼苗叶片膜脂过氧化及质子泵活性的影响[J]. 田婧,郭世荣,孙锦,王丽萍,阳燕娟,李斌. 应用生态学报. 2011(12)
本文编号:3640443
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