Genetic and agronomic traits stability of marker-free transg

发布时间：2023-04-16 20:10

　　Genetically modified wheat has not been commercially utilized in agriculture largely due to regulatory hurdles associated with traditional transformation methods. Development of marker-free transgenic wheat plants will help to facilitate biosafety evaluation and the eventual environmental release of transgenic wheat varieties. In this study, the marker-free transgenic wheat plants previously obtained by Agrobacterium-mediated co-transformation of double T-DNAs vector were identified by fluoresce...

【文章页数】：10 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials
    2.2. Histochemical staining and Quickstix detection of bar expression in transgenic wheat plants
    2.3. DNA extraction and PCR amplification
    2.4. Fluorescence in situ hybridization
    2.5. Examination of agronomic traits and statistical analysis
3. Results
    3.1. Identification of T1 transgenic wheat plants by FISH
    3.2. Genetic stability of the marker-free transgenic wheat plants in the T2 generation
    3.3. Comparison of main agronomic traits between T3 transgenic lines and wild type
4. Discussion
    4.1. Importance and strategies of obtaining marker-free transgenic plants
    4.2. Application of FISH technology in identification of transgenic plants
    4.3. Potential of generating marker-free gene editing wheat plants
    4.4. Influence of transgene integration on main agronomic traits in transgenic wheat plants
5. Conclusion



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