Improving the efficiency of the CRISPR-Cas12a system with tR
发布时间：2023-06-03 14:23
CRISPR-Cas12a offers a convenient tool for multiplex genome editing in rice. However, the CRISPR-Cas12a system displays variable editing efficiency across genomic loci, with marked influence by CRISPR RNAs(crRNAs). To improve the efficiency of the CRISPR-Cas12a system for multiplex genome editing, we identified various architectures and expression strategies for crRNAs. Transformation of binary vectors loaded with engineered CRISPR-Cas12a systems into rice calli and subsequent sequencing reveale...
【文章页数】：5 页
【文章目录】：
1. Introduction
2. Materials and methods
2.1. Screening and designing crRNAs
2.2. Binary vector construction and generation of transgenic rice
2.3. Detection of mutations
3. Results
3.1. Longer crRNAs exert different effects on rice genome editing efficiencies
3.2. A modified tRNA-crRNA array is efficient for multiplex genome editing in rice
3.3. Modified tRNA-crRNA arrays overcome the failure of genome editing
4. Discussion
Declaration of Competing Interest
Appendix A.Supplementary data
本文编号：3829503
【文章页数】：5 页
【文章目录】：
1. Introduction
2. Materials and methods
2.1. Screening and designing crRNAs
2.2. Binary vector construction and generation of transgenic rice
2.3. Detection of mutations
3. Results
3.1. Longer crRNAs exert different effects on rice genome editing efficiencies
3.2. A modified tRNA-crRNA array is efficient for multiplex genome editing in rice
3.3. Modified tRNA-crRNA arrays overcome the failure of genome editing
4. Discussion
Declaration of Competing Interest
Appendix A.Supplementary data
本文编号：3829503
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