Evaluation of drought tolerance in ZmVPP1-overexpressing tra

发布时间：2024-01-14 10:23

　　The vacuolar proton-pumping pyrophosphatase gene(VPP) is often used to enhance plant drought tolerance via genetic engineering. In this study, the drought tolerance of four transgenic inbred maize lines overexpressing ZmVPP1(PH4 CV-T, PH6 WC-T, Chang7-2-T, and Zheng58-T) and their transgenic hybrids was evaluated at various stages. Under normal and drought conditions, the height and fresh weight were greater for the four transgenic inbred maize lines than for the wild-type(WT) controls at the ge...

【文章页数】：11 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Transgenic inbred lines and hybrids
    2.2. Total RNA extraction and analysis of Zm VPP1expression
    2.3. Screening for drought tolerance at the germination stage
    2.4. Assessment of drought tolerance at the seedling stage
    2.5. Evaluation of the drought tolerance of transgenic inbred lines and hybrids under field conditions
    2.6. Statistical analysis
3. Results
    3.1. Relative Zm VPP1 expression in transgenic inbred maize lines
    3.2. Evaluation of the drought tolerance of transgenic inbred lines at the germination stage
    3.3. Increased drought tolerance and photosynthesis in the transgenic inbred lines at the seedling stage
    3.4. Ear weight of transgenic inbred maize lines and hybrids under field conditions
4.Discussion
    4.1. Overexpression of Zm VPP1
    4.2. Overexpression of Zm VPP1 gene promoted photosynthesis of maize
    4.3. Utilization of transgenic inbred lines
5. Conclusion



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