慢性缺氧改变外周血转录组模式（英文）

发布时间：2021-06-21 09:31

　　目的多种生理和病理状态都会伴随着慢性缺氧,例如紫绀型先天性心脏病（CCHD）。这种慢性缺氧可能会干扰基因的转录过程。然而,在缺氧条件下外周血转录组模式的改变尚无报道。故本工作旨在探讨慢性缺氧条件下,外周血转录组模式的变化。方法本研究使用慢性缺氧大鼠模型模拟CCHD患者的低氧状态。两组Sprague-Dawley大鼠（每组n=6）分别暴露于低氧（10%O2）或常氧（21%O2）条件下饲养3周。每周测量大鼠体重。缺氧处理结束后,采集两组大鼠外周血并提取总RNA进行RNA-Seq。经过质量评估后,通过Illumina Hiseq平台对文库进行测序。筛选差异表达基因（DEG）,筛选条件为FDR（false discovery rate）<0.05且FC（fold change）>2。进行DEG的功能注释和聚类分析,并选取出padj（adjusted P-value）<0.05的条目。结果低氧组大鼠体重较对照组明显降低（P<0.01）。RNA-Seq结果表明:两组的转录组模式有显著差异。共鉴定出了872个差异表达的基因。在缺氧组中,共有803个基因下调,只有69个基因上调... 

【文章来源】：Chinese Medical Sciences Journal. 2020,35(01)CSCD

【文章页数】：12 页

【文章目录】：
MATERIALS AND METHODS
    Study design and animal model
    Blood sampling and RNA extraction
    RNA library preparation and deep sequencing
    Quality control and reads mapping
    Quantification of gene expression level and differential expression analysis
    Functional annotation and enrichment of differentially expressed genes
    Protein-Protein Interaction analysis of DEGs
    Statistical analysis
RESULTS
    The characteristics of the studied rats
    Quality of RNA-Seq analysis
    Differential gene expression analysis between two groups
    Functional enrichment analysis of DEGs
    Gene interaction network and functional modules
DISCUSSION
Conflict of Interests Statement



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