拟南芥响应高钙的miRNAs的筛选与鉴定（英文）

发布时间：2021-08-29 08:01

　　喀斯特地区土壤中钙含量较高。土壤钙含量过高会抑制植物生长,进而影响农作物产量和质量。目前,对植物应答和耐受高钙胁迫的机理研究还不深入。miRNAs在植物对生物和非生物胁迫的响应中起重要调控作用。本文应用转录组测序,对高钙响应拟南芥根部miRNAs进行分析,筛选到受高钙胁迫显著影响的12个miRNAs,并对其靶基因进行了预测和分析。实时定量PCR分析证实,大部分差异表达miRNAs和其靶基因表达负相关。本研究为进一步揭示miRNAs在植物响应高钙胁迫过程中的调控作用奠定了基础。 

【文章来源】：生命科学研究. 2020,24(01)CSCD

【文章页数】：8 页

【文章目录】：
1 Materials and methods
    1.1 Plant growth and high calcium treatment
    1.2 Total RNA extraction and small RNA iso-lation and sequencing
    1.3 Bioinformatics analysis of miRNA sequen-cing data
    1.4 Differential expression analysis of miRNAs
    1.5 Prediction and annotation of target genes for differentially expressed miRNAs
    1.6 RT-qPCR analysis of high calcium-res-ponsive miRNAs and their predicted target genes
2 Results
    2.1 High-throughput sequencing results of sm-all RNA libraries from Arabidopsis roots
    2.2 Differential expression analysis of Ara-bidopsis miRNAs under high calcium stress
    2.3 Target prediction and annotation of differ-entially expressed miRNAs
    2.4 RT-qPCR analysis of high calcium-re-sponsive miRNAs and their target genes
3 Discussion
Supplement data



本文编号：3370242