开颅法制作大鼠局灶性脑缺血再灌注模型的改良研究

发布时间：2018-03-03 20:37

本文选题：脑缺血　切入点：再灌注损伤　出处：《神经解剖学杂志》2016年05期 　论文类型：期刊论文

【摘要】：目的:改良传统的开颅造模法,建立一种稳定、可靠的大鼠脑缺血再灌注模型。方法:20只SD大鼠随机分为假手术与缺血再灌注组,每组10只,分别行假手术操作及改良模型制作。改良模型为,大鼠开颅后暴露左侧大脑中动脉(middle cerebral artery,MCA),将直径0.12 mm的不锈钢丝放置于MCA下,近其主干,钢丝两端架于颞骨表面120 min,撤去钢丝即再灌注。假手术仅暴露MCA,不进行缺血及再灌注。大鼠再灌注24 h,通过神经功能缺失评分,TTC染色,神经元计数及病理形态学的方法对模型进行评价。结果:改良后的造模成功率为100%,梗死灶位于额、顶叶皮层,病理形态学表现为典型的缺血性改变,正常神经元数减少(P0.01)。神经功能缺失评分1.3±0.5,脑梗死体积比率为5.32%±1.28%,与假手术组相比均有统计学意义(P0.01)。结论:这一改良模型阻断及恢复MCA血流明确,梗死灶的大小、位置稳定,死亡率低,为脑缺血再灌注机制的研究及治疗方法的探讨提供了有益帮助。
[Abstract]:Objective: to establish a stable and reliable rat model of cerebral ischemia-reperfusion by modified craniotomy. Methods: twenty Sprague-Dawley rats were randomly divided into sham-operation group and ischemia-reperfusion group with 10 rats in each group. After craniotomy, the middle cerebral artery of left middle cerebral artery was exposed, and the stainless steel wire with diameter of 0.12 mm was placed under MCA. The two ends of the wire were placed on the surface of the temporal bone for 120 min. The wire was removed and then reperfusion. The sham operation only exposed MCA without ischemia or reperfusion. The rats were subjected to 24 h reperfusion and were stained with TTC by neurological impairment score. Results: the success rate of the improved model was 100. The infarct was located in the frontal cortex and parietal cortex. The number of normal neurons decreased (P 0.01). The neurological deficit score was 1. 3 卤0. 5, and the volume ratio of cerebral infarction was 5.32% 卤1. 28. Compared with the sham operation group, there was significant difference between the two groups (P 0. 01). Conclusion: this improved model can block and restore MCA blood flow clearly, and the infarct size and location are stable. The low mortality rate provides useful help for the study of cerebral ischemia reperfusion mechanism and therapy.
【作者单位】：上海中医药大学药物安全评价研究中心;上海中医药大学实验动物中心;
【基金】：国家自然科学基金(81574078,81072646)
【分类号】：R743.3;R-332

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