乙醇沉淀法制备不同人血白蛋白微球的研究

发布时间：2018-05-20 16:11

  本文选题：乙醇沉淀法 + 人血白蛋白　； 参考：《长春中医药大学》2011年硕士论文

【摘要】：目的:考察不同的制备人血白蛋白微球的方法,选择适于工业化生产的制备方法,研究该方法制备不同粒径人血白蛋白微粒的最佳条件,并对于其装载人参皂苷Rb1、阿霉素、异硫氰酸荧光素、银耳多糖4种性质不同药物时方法的适用性、制备的微球性质进行考察。 方法:采用乙醇沉淀法制备不同粒径的微粒,采用吸附法和结合法装载不同种类的药物。使用HPLC法测定人参皂苷Rb1、阿霉素、异硫氰酸荧光素的载药量,采用分光光度计法测定银耳多糖的载药量。并通过透析法、酶解法、冻干法考察人参皂苷Rb1人血白蛋白微球的释药性能、体外降解性能和储存工艺。 结果:采用乙醇沉淀法制备出3种粒径区间的微粒,分别为:“100nm以下”、“400nm-1000nm”、“1μm-2μm”。并可以装载人参皂苷Rb1、阿霉素、异硫氰酸荧光素、银耳多糖。载人参皂苷Rb1和银耳多糖的人血白蛋白微粒载药量较高,载阿霉素和异硫氰酸荧光素的人血白蛋白微粒载药量较低。人参皂苷Rb1人血白蛋白微球具有明显的缓释性能,可调节其体外降解时间,冻干后在4℃下稳定的保存6个月。 结论:适宜采用乙醇沉淀法制备成载人参皂苷Rb1和银耳多糖的人血白蛋白微粒,这些药物水溶性好、化学性质稳定、药物性质为中性,并可按照需要制备不同粒径、不同载药量、不同降解时间的微粒。不适宜制备载阿霉素等具有较活泼化学集团、可使蛋白变性的药物。对异硫氰酸荧光素等水溶性较差药物,本方法也有一定的局限性。
[Abstract]:Objective: to investigate the different preparation methods of human serum albumin microspheres, to select the suitable preparation method for industrial production, to study the optimum conditions for the preparation of different particle sizes of human serum albumin microspheres, and to study the loading of ginsenoside Rb1, doxorubicin for the preparation of human serum albumin microspheres. The applicability of four different methods for the preparation of fluorescein isothiocyanate and Tremella polysaccharide were investigated. Methods: different particle sizes were prepared by ethanol precipitation, and different kinds of drugs were loaded by adsorption and binding methods. The drug loading of ginsenoside Rb1, doxorubicin and fluorescein isothiocyanate was determined by HPLC, and the drug loading of Tremella polysaccharide was determined by spectrophotometer. The drug release, in vitro degradation and storage of ginsenoside Rb1 human albumin microspheres were investigated by dialysis, enzymatic hydrolysis and lyophilization. Results: three kinds of particle sizes were prepared by ethanol precipitation, which were "below 100nm", "400nm-1000nm" and "1 渭 m -2 渭 m". Ginsenoside RB 1, adriamycin, fluorescein isothiocyanate, Tremella polysaccharide can be loaded. The amount of human serum albumin particles loaded with ginsenoside Rb1 and Tremella polysaccharide was higher than that with doxorubicin and fluorescein isothiocyanate. Ginsenoside Rb1 human serum albumin microspheres have obvious slow-release properties, and can adjust the degradation time in vitro. After freeze-drying, the microspheres are kept steadily at 4 鈩，

本文编号：1915348