魔芋飞粉中ACE抑制肽酶法制备技术研究
发布时间:2018-06-01 09:02
本文选题:酶法 + 魔芋飞粉 ; 参考:《食品工业》2016年12期
【摘要】:以魔芋飞粉为原料制备血管紧张素转换酶(ACE)抑制肽,为魔芋飞粉的开发利用,生产高附加值产品开辟一条新的途径。以体外ACE抑制率为指标筛选最佳用酶并确定酶解条件。结果表明:胃蛋白酶为酶解魔芋飞粉最佳用酶,其最佳酶解条件为:底物浓度2%(净蛋白),酶用量5 000 U/g底物蛋白,温度37℃,pH 2.5,酶解时间210 min,此时酶解液的ACE抑制率为92.65%,多肽得率为12.60%。魔芋飞粉经蛋白酶酶解可制备高活性的ACE抑制肽。
[Abstract]:Angiotensin converting enzyme ( ACE ) was used as raw material to prepare angiotensin converting enzyme ( ACE ) inhibitory peptide . The best enzyme was selected for the development and utilization of konjac fly powder . The best enzyme was used to screen the best enzyme . The best enzymolysis conditions were as follows : substrate concentration 2 % ( net protein ) , enzyme dosage of 5 000 U / g substrate protein , temperature 37 鈩,
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