酶解-交联法制备多孔淀粉及其负载红景天苷研究

发布时间：2018-06-05 07:22

本文选题：酶解法 + 三偏磷酸钠　；参考：《食品工业科技》2017年03期

【摘要】：以玉米淀粉为原料,采用α-淀粉酶和糖化酶复合酶解与三偏磷酸钠交联改性相结合的方法制备了一系列具有不同交联度的交联多孔淀粉。通过各种表征手段考察了原玉米淀粉、多孔淀粉和交联多孔淀粉的形态结构和理化性质,并应用于负载红景天苷研究。该方法制备的交联多孔淀粉具有良好的蜂窝状中空结构,有利于淀粉颗粒对目的物质的吸附。相比原淀粉和未交联多孔淀粉,交联多孔淀粉的吸油率有较大提高,对红景天苷的包封率为58.89%。经过交联反应后的交联多孔淀粉不但晶型没有发生改变,还具有更高的总孔面积、平均孔直径和孔隙率值,其分子结构也更加紧凑,更具刚性和更高的糊化温度,说明交联改性提高了多孔淀粉的结构性能。体外释放结果表明载药交联多孔淀粉具有一定的缓释性能,能够提高红景天苷的生物半衰期和生物利用度。
[Abstract]:A series of cross-linked porous starch with different crosslinking degree were prepared by combining 伪 -amylase and Glucoamylase with sodium trimetaphosphate crosslinking modification with corn starch as raw material. The morphology, structure and physicochemical properties of corn starch, porous starch and cross-linked porous starch were investigated by various characterization methods, and were applied to the study of salidroside loading. The cross-linked porous starch prepared by this method has a good honeycomb hollow structure, which is beneficial to the adsorption of starch particles to the target substance. Compared with raw starch and uncross-linked porous starch, the oil absorption rate of cross-linked porous starch was greatly improved, and the entrapment efficiency of salidroside was 58.89. After crosslinking, the cross-linked porous starch not only has no change in crystal form, but also has higher total pore area, average pore diameter and porosity value, and its molecular structure is also more compact, more rigid and higher gelatinization temperature. The structure and properties of porous starch were improved by crosslinking modification. The results of release in vitro showed that cross-linked porous starch containing drugs had a certain sustained release performance and could improve the biological half-life and bioavailability of salidroside.
【作者单位】：南昌大学食品科学与技术国家重点实验室;南昌大学化学工程系;
【基金】：国家自然科学基金委员会资助项目(31160317) 江西省自然科学基金(2014BAB213003和20151BAB203029)
【分类号】：TS231

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