pH调节法制备河蚬蛋白质及其营养评价
发布时间：2018-07-17 04:09
【摘要】：以河蚬为原料,采用p H调节法(p H-shifting)分离提取河蚬蛋白质并对其进行营养评价。通过单因素和响应面法(RSM)试验优化碱溶蛋白质提取工艺,确定最佳条件为:p H12.5、液料比44.3:1(v/w)、提取温度67.2℃、提取时间60 min,河蚬蛋白质提取率达74.97%。采用酸沉碱溶蛋白质,最佳条件为p H4.5。在最佳分离提取条件下获得的河蚬分离蛋白质得率为62.51%,蛋白质纯度达91.62%。分离制备的河蚬蛋白质必需氨基酸与非必需氨基酸比值(EAA/NEAA)和必需氨基酸与总氨基酸比值(EAA/TAA)分别为88.55%和41.55%,均高于FAO/WHO推荐的理想蛋白模式,必需氨基酸指数(EAAI)和氨基酸比值系数分(SRCAA)分别为86.54和82.02。研究结果表明p H调节法可应用于分离制备较高得率和营养价值的蛋白质。研究结果为制备河蚬分离蛋白质和进一步开发利用河蚬蛋白质提供了理论依据和实践基础。
[Abstract]:The protein of Corbicula fluminea was isolated by pH-shifting method and its nutrition was evaluated. Single factor and response surface method (RSM) were used to optimize the extraction process of alkali-soluble protein. The optimum conditions were determined as follows: ratio of liquid to material 44.3: 1 (v / w), extraction temperature 67.2 鈩，
本文编号：2128951
[Abstract]:The protein of Corbicula fluminea was isolated by pH-shifting method and its nutrition was evaluated. Single factor and response surface method (RSM) were used to optimize the extraction process of alkali-soluble protein. The optimum conditions were determined as follows: ratio of liquid to material 44.3: 1 (v / w), extraction temperature 67.2 鈩，
本文编号：2128951
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