应激性抑郁样行为发生中眶额叶5-HT1A受体对谷氨酸和γ-氨基丁酸的调节

发布时间：2018-01-10 02:25

本文关键词：应激性抑郁样行为发生中眶额叶5-HT1A受体对谷氨酸和γ-氨基丁酸的调节　出处：《心理学报》2015年10期 　论文类型：期刊论文

【摘要】：5-羟色胺(5-hydroxytryptamine,5-HT)在抑郁症的发生及治疗中起着极其重要的作用,谷氨酸(glutamic acid,Glu)过度释放是应激性抑郁发生的重要原因。为了证明应激性抑郁样行为发生中眶额叶(orbitofrontal cortex,OFC)5-HT1A受体是否通过对Glu和γ-氨基丁酸(gamma-aminobutyric acid,GABA)的调节而发挥作用。本研究通过建立慢性不可预见性温和应激(chronic unpredictable mild stress,CUMS)抑郁模型,结合OFC区分别微量注射5-HT1A受体激动剂8-OH-DPAT和拮抗剂WAY100635,采用糖水偏爱率测试、旷场测试及悬尾测试检测动物的行为学表现,并通过高效液相色谱法(high-performance liquid chromatography,HPLC)分别检测了OFC区5-HT、Glu和GABA的含量。结果显示,CUMS组和注射WAY100635组大鼠较对照组表现出明显的抑郁样行为,且Glu含量显著升高,但5-HT水平和GABA含量均未有显著性变化。微量注射8-OH-DPAT后,能够显著改善大鼠的抑郁样行为,且Glu显著降低,但5-HT水平和GABA含量仍未发生显著性变化。结果表明,慢性不可预见性温和应激所引起的抑郁样行为,并不是由于眶额叶5-HT水平的降低,而可能是由于5-HT不能满足应激状态下调节Glu能神经的需要,或可能是OFC区Glu能神经元上5-HT1A受体功能的降低,导致Glu水平过高所致。眶额叶5-HT1A受体在Glu合成与释放中发挥重要调节作用。
[Abstract]:Serotonin 5-hydroxytryptamine 5-HT plays an extremely important role in the occurrence and treatment of depression, glutamic acid acid. In order to prove that orbitofrontal cortex is involved in the occurrence of stress-like depressive behavior, excessive release of Glu) is an important cause of stress-induced depression. Whether OFC)5-HT1A receptors pass through Glu and gamma-aminobutyric acid. In this study, chronic unpredictable mild stress was established by establishing chronic unpredictable mild stress. CUMS depression model, 5-HT1A receptor agonist 8-OH-DPAT and antagonist WAY100635 were microinjected into the OFC region, respectively. The sugar water preference rate was measured. The behavior of animals was measured by open field test and tail suspension test, and high-performance liquid chromatography was performed by high performance liquid chromatography (HPLC). The contents of 5-HT-Glu and GABA in the OFC region were determined by HPLC, respectively. Compared with the control group, the CUMS group and the WAY100635 injection group showed obvious depressive behavior, and the content of Glu increased significantly. However, the level of 5-HT and the content of GABA did not change significantly. After microinjection of 8-OH-DPAT, the depressive behavior of rats was significantly improved, and Glu was significantly decreased. The results showed that depression induced by chronic unpredictable mild stress was not due to the decrease of 5-HT level in the orbital frontal lobe. It may be that 5-HT can not meet the need of regulating Glu neurons under stress, or that the function of 5-HT1A receptor in Glu neurons in OFC region is decreased. 5 HT 1A receptor plays an important role in the synthesis and release of Glu.
【作者单位】：陕西师范大学生命科学学院;
【基金】：国家自然科学基金资助项目(81371512)
【分类号】：B845
【正文快照】： 1问题提出抑郁症是一种比较普遍,且危害极大的心理与精神障碍性疾病。单胺类递质失调学说是研究抑郁症发生机制及治疗的主要理论之一。基于此而被广泛使用的一些抗抑郁药虽能快速增加突触间隙单胺类递质的浓度,但由于其抗抑郁起效时间延迟,使得单胺类递质失调假说不足以解释抑

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