广西南丹少数民族地区人群慢性心理应激相关心理和生理指标关系的研究

发布时间：2018-06-30 09:13

本文选题：少数民族 + 慢性心理应激　；参考：《广西医科大学》2017年硕士论文

【摘要】：目的1.了解广西南丹少数民族地区白裤瑶、壮族和汉族人群心理应激相关血清5-羟色胺(5-HT)、c-fos蛋白水平和神经肽S受体1(NPSR1)的含量水平。2.了解广西南丹地区三个民族的心理应激水平及相关因素对血清水平的影响,并在此基础上探索研究对象慢性心理应激作用下心理和生理的关系。方法1.采用分层随机整群抽样法,在广西南丹白裤瑶族聚居地里湖乡、八圩乡抽取131名白裤瑶族,同时抽取附近村落的111名汉族和124名壮族作为对照,共366名不同民族的村民作为调查对象。2.采用自编一般人口学资料调查问卷、简化症状自评量表、生活事件量表(LES)、简易应对方式量表和社会支持评定量表(SSRS)对研究对象进行问卷调查。3.采集被调查者空腹静脉血,采用酶联免疫吸附测定(ELISA)检测血清中5-羟色胺(5-HT)、c-fos蛋白和神经肽S受体1(NPSR1)的含量。利用SPSS 21.0进行统计分析。应用t检验、卡方检验和方差分析进行慢性心理应激及其影响因素与细胞应激水平之间的关联性分析。应用多元线性回归模型分析慢性心理应激对相关血清指标等因素的影响。应用层次回归模型来分析目标影响因素对慢性心理应激的调节效应。结果1.研究对象慢性心理应激的情况:本次调查对象的样本les总分25.23±29.56,阳性率43.99%;心理症状总分42.43±12.26,总体阳性率12.73%;应对总分40.46±11.51,积极应对25.62±8.79,消极应对14.83±4.35;社会支持总分27.76±6.20,主观支持12.67±3.32,客观支持8.23±2.64,支持利用度6.86±2.39;三民族在焦虑因子和积极应对上存在差异(f=3.475~5.506,p0.05)。2.研究对象的血清指标水平:本次研究对象血清c-fos蛋白水平(223.34±31.47)pg/ml;5-ht水平(281.57±18.15)pg/ml;npsr1水平(182.65±13.48)pg/ml。3.应激相关血清生理学指标与其他影响因素之间的关系研究:血清c-fos蛋白水平在不同生活事件和抑郁水平上差异有统计学意义(t=1.974~3.489,p0.05);血清5-ht水平在不同性别、生活事件、社会支持、消极应对和应激性情绪反应水平上差异有统计学意义(t=2.005~10.997,p0.05);血清npsr1水平在不同家庭人均收入、生活事件、消极应对和抑郁水平上差异有统计学意义(t或f=2.017~5.522,p0.05)。多元回归分析显示,消极应对、血清5-ht水平为广西南丹少数民族地区慢性心理应激水平的影响因素(β=0.599,-1.537;p0.05)。4.研究因素的交互分析:慢性心理应激心理、生理相关因素的交互分析显示,消极应对和5-ht对生活事件总分作用于心理健康具有显著的调节作用(r2=0.512~0.701,p0.05)。结论1.慢性心理应激水平可导致应激相关血清指标水平的不同。2.应激相关血清指标水平受民族、性别、家庭收入、应激性情绪反应、消极应对和生活事件水平等因素的影响。
[Abstract]:Objective 1. To investigate the level of 5-HT c-fos protein and the level of neuropeptide S receptor 1 (NPSR1) in the psychological stress related to psychological stress of Baikouyao, Zhuang and Han nationality in Nandan minority area of Guangxi. To understand the level of psychological stress and the influence of related factors on serum level of three nationalities in Nandan, Guangxi, and to explore the relationship between psychology and physiology under chronic psychological stress. Method 1. Using stratified random cluster sampling method, 131 Bai Kuyao people were collected from Lihu Township, Bawei Township, Nandan County, Guangxi, and 111 Han nationality and 124 Zhuang nationality from nearby villages were selected as control. A total of 366 villagers from different nationalities were investigated. The self-designed questionnaire of general demographic data, simplified symptom Checklist, Life events scale (les), simple coping style scale and Social support rating scale (SSRS) were used to investigate the subjects. Fasting venous blood was collected and the levels of serotonin (5-HT) c-fos protein and neuropeptide S receptor 1 (NPSR1) in serum were determined by enzyme-linked immunosorbent assay (Elisa). SPSS 21.0 was used for statistical analysis. T test, chi-square test and variance analysis were used to analyze the relationship between chronic psychological stress and its influencing factors. Multivariate linear regression model was used to analyze the influence of chronic psychological stress on serum indexes. A hierarchical regression model was used to analyze the regulatory effects of target influencing factors on chronic psychological stress. Result 1. Subjects' chronic psychological stress: the total score of les was 25.23 卤29.56, the positive rate was 43.99; the total score of psychological symptoms was 42.43 卤12.26, the total positive rate was 12.73; the total score of coping was 40.46 卤11.51, the positive response was 25.62 卤8.79, the negative was 14.83 卤4.35; the total score of social support was 27.76 卤6.20; the total score of social support was 27.76 卤6.20, and the total score of subjective support was 27.76 卤6.20. 12.67 卤3.32, objective support 8.23 卤2.64, support utilization 6.86 卤2.39.The differences in anxiety factor and positive coping were found among the three nationalities (fen 3.4755.506p0.05) .2. The serum levels of c-fos protein were (223.34 卤31.47) PG / ml ~ (5-ht) ~ (281.57 卤18.15) PG / ml ~ (-1) and (182.65 卤13.48) PG / ml 路ml ~ (-1) respectively. Study on the relationship between stress related Serum physiological Indexes and other influencing factors: there were significant differences in serum c-fos protein levels between different life events and depression levels (t 1.974 + 3.489 p0.05), and serum 5-ht levels were different in gender, life events and social support. There were significant differences in the level of negative coping and stress emotional response (t = 2.005, 10.997p0.05), and in the level of serum npsr1 in different families, such as per capita income, life events, negative coping and depression (t or fen 2.017 卤5.522p0.05). Multiple regression analysis showed that negative coping and serum 5-ht level were the influencing factors of chronic psychological stress in Nandan ethnic minority area of Guangxi (尾 -0.599- 1.537p0.05) .4. The interaction analysis of chronic psychological stress and physiological related factors showed that negative coping and 5-ht had significant regulating effect on mental health (r _ 2 / 0. 512 / 0.701p _ (0.05). Conclusion 1. Chronic psychological stress level can lead to different levels of stress-related serum indicators. 2. 2. The serum levels of stress-related indexes were influenced by nationality, sex, family income, stress emotional response, negative coping and life event level.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：B842

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