Characterization of the Complete Mitochondrial Genomes of Ne

发布时间：2021-12-16 22:48

　　胃肠道寄生虫给全世界的畜牧业造成重大经济损失。其中,奥斯特线虫是寄生于反刍动物皱胃中的一种红棕色的线虫,导致胃炎（奥斯特线虫病）,全世界范围内广泛流行,尤其是在温带气候地区。寄生线虫病是很容易被忽视并且对人类的健康造成了巨大的威胁。据估计,最常见的土源性寄生线虫为鞭虫（毛首鞭形线虫）,钩虫（十二指肠钩口线虫和美洲钩虫）以及蛔虫（蛔虫）等线虫广泛流行于世界较贫穷地区,感染人数达到十亿人。线粒体是有重要生化功能的细胞器,是真核细胞产生能量的场所。线粒体（mt）基因组位于细胞器内且独立于核基因组,但彼此之间具有密切的关系。本研究对小反刍动物寄生线虫奥斯特线虫的完整线粒体（mt）基因组进行了DNA序列分析,并在预测的线粒体氨基酸序列数据集的基础上,对其与旋毛虫超家族成员的系统发育关系进行了研究。奥斯特线虫的整个线粒体基因组是圆形的,长度为14,151bp。它由总共36个基因组成,包括12个编码蛋白质的基因（PCGs）,2个核糖体RNA（rRNA）基因,22个转移RNA（tRNA）基因和2个非编码区域,所有的基因都在同一方向转录。进化分析结果显示,基于12个蛋白质编码基因的预测氨基酸序列与捻转血... 

【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校

【文章页数】：135 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
Chapter 1 Review of Literature
    1.1 Background
    1.2 Progressive goat industry in China
    1.3 Parasitology
        1.3.1 Ostertagia spp
        1.3.2 Trichuris spp
    1.4 Overview of mitochondria and mt genome sequencing
        1.4.1 Mitochondria
        1.4.2 Evolution of mitochondria
        1.4.3 Mitochondrial Gene Content of Animals
        1.4.4 Mitochondrial Genome Structure
    1.5 Molecular Biology
        1.5.1 Phylogenetic studies
    1.6 Next generation sequencing(NGS)technology and parasite genomics
    1.7 Parasite Genomes
        1.7.1 Mitochondrial genomes of parasites
        1.7.2 Workflow based on NGS for mitochondrial genome
    Objectives
Chapter 2 Characterization of the Complete Mitochondrial Genome of Ostertagia trifurcata of Small Ruminants and its Phylogenetic Associations for the Trichostrongyloidea Superfamily
    1.Introduction
    2.Materials and Methods
        2.1 Collection of worms and extraction of DNA
        2.2 Amplification of ITS-2 of Ostertagia trifurcata
        2.3 Amplification of long fragments and sequencing
        2.4 Gene annotation and sequence analysis
        2.5 Phylogenetic analysis on basis of dataset of amino acid sequences
    3.Results and Discussion
        3.1 ITS-2 analysis
        3.2 Organization,content and mt genome annotation
        3.3 Phylogenetic analysis
    4.Implications and Significance
    5.Conclusion
Chapter 3 Characterization of the complete mitochondrial genome of a whipworm Trichuris skrjabini(Nematoda:Trichuridae)
    1.Introduction
    2.Materials and Methods
        2.1 Parasite collection and isolation of DNA
        2.2 Trichuris skrjabini ITS-2 Amplification
        2.3 Amplification of short and long fragments by PCR
        2.4 Gene annotation and sequence analysis
        2.5 Phylogenetic analysis
    3.Results
        3.1 ITS-2 analysis
        3.2 Amplification of short and long fragments
        3.3 mt Genome Features
        3.4 Annotation
        3.5 Phylogenetic analysis
    4.Discussion
    5.Conclusion
Chapter4 SUMMARY
References
Appendix
    ITS-2 sequence of Ostertagia trifurcata
    Whole mt genome sequence of Ostertagia trifurcata(Accession No.MK227249)
    Features of Osteragai trifurcata mt genome
    ITS-2 sequence of Trichuris skrjabini
    Partial sequences of Trichuris skrjabini mitochondrial genes
    Whole mt genome sequence of Trichuris skrjabini(Accession No.MK333462)
    Features of Trichuris skrjabini mt genome
Acknowledgements
CURRICULUM VITAE


【参考文献】：
期刊论文
[1]Herbal traditional Chinese medicine and its evidence base in gastrointestinal disorders[J]. Rolf Teschke,Albrecht Wolff,Christian Frenzel,Axel Eickhoff,Johannes Schulze.  World Journal of Gastroenterology. 2015(15)



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