不同强度的运动对大鼠心肺耐力及PGC-1α通路的影响

发布时间：2018-04-24 21:52

本文选题：心肺耐力 + 有氧运动　；参考：《北京体育大学》2015年博士论文

【摘要】：研究目的：通过12周不同强度的运动干预,观察大鼠心肺耐力的改善效果及心肌、骨骼肌PGC-1α通路的变化特点和规律,探讨心肺耐力提高的可能机制。研究方法：40只雄性Wistar大鼠随机分为安静组、小强度组(50% VO2 max)、中强度组(65% VO2 max)、大强度组(80% VO2 max),每组10只。适应性训练3天后测试最大摄氧量,之后每隔两周测试一次。运动组大鼠每天训练1h,每周5天。12周运动干预后,进行超声心动测试。于最后一次运动后48h麻醉后腹主动脉取血、心肌和右侧比目鱼肌。RT-qPCR法测定PGC-lα、AMPK、SIRT1、 NRF1、NRF2、ERRα mRNA表达。Western Blot法测定PGC-1α、AMPK、磷酸化AMPK、SIRT1蛋白表达。研究结果：1、三种强度的运动都能减少体重增加的趋势。其中大强度组体重增加最少。三个运动组对VO2max的提升都很有效,小强度组VO2max的提升需要更长时间。2、三种强度的运动都能改善血脂。只有大强度运动可以显著改善心脏功能。3、运动组心肌和比目鱼肌PGC-1α mRNA表达增加,但只有中强度组比目鱼肌PGC-1α蛋白表达显著增加。4、运动强度的改变对AMPK蛋白表达影响不大,但随着运动强度增加,pAMPK和SIRT1蛋白表达都有所增加。5、中强度组和大强度组心肌NRF1 mRNA表达增加,但三个运动组比目鱼肌NRF1 mRNA却低于安静组。运动组NRF2 mRNA低于安静组。6、中强度和大强度运动可以诱导心肌ERRα mRNA表达。比目鱼肌中ERRα mRNA的变化与心肌不同,大强度组最低。研究结论：(1)12周的运动干预可明显改善大鼠的心肺耐力。中强度运动和大强度运动提升效果较快,分别在第八周末和第六周末达到峰值。而小强度组在第十周末明显提升,并于十二周末达到峰值。(2)12周的运动干预可以减小大鼠体重增长的幅度。大强度运动体重增长最少,其心脏泵血功能的改善最显著。(3)运动改善心肺耐力的分子机制主要是通过增加骨骼肌PGC-1α的表达而不是心肌。中强度运动和大强度运动对心肌和骨骼肌AMPK和SIRT1的活化有显著影响,尤其是中强度运动。(4)三个运动组AMPK、SIRT1、PGC-1α的mRNA表达不能反映各自蛋白表达水平,转录后的调控可能起重要作用。
[Abstract]:Objective: to observe the improvement of cardiopulmonary endurance and the changes of PGC-1 伪 pathway in myocardium and skeletal muscle of rats after 12 weeks of exercise with different intensities, and to explore the possible mechanism of improving cardiopulmonary endurance. Methods Forty male Wistar rats were randomly divided into quiet group (n = 10), small strength group (n = 10), medium strength group (n = 10), middle strength group (n = 65) and high intensity group (n = 10). The maximal oxygen uptake was tested after 3 days of adaptive training and then every two weeks. The rats in the exercise group were trained for 1 hour, 5 days a week and 12 weeks after exercise intervention, and then the echocardiography test was carried out. After 48 hours of anaesthesia, blood was taken from abdominal aorta. The expression of PGC-l 伪 -AMPK-SIRT1 in myocardium and right soleus muscle was determined by RT-qPCR, and the expression of PGC-1 伪 -AMPKand phosphorylated AMPK-SIRT1 protein was detected by Western Blot. The study found that all three strenuous exercises reduced the trend of weight gain. The weight gain was the least in the high intensity group. All of the three exercise groups were effective in improving VO2max, and the increase of VO2max in the small intensity group required a longer period of time. All three kinds of intensity exercise could improve blood lipids. The expression of PGC-1 伪 mRNA in myocardium and soleus muscle increased significantly in exercise group, but the expression of PGC-1 伪 protein in soleus muscle increased significantly in moderate intensity group. The change of exercise intensity had little effect on the expression of AMPK protein. However, the expression of pAMPK and SIRT1 protein increased with the increase of exercise intensity, and the expression of NRF1 mRNA increased in moderate and high intensity groups, but NRF1 mRNA in soleus muscle of three exercise groups was lower than that in quiet group. NRF2 mRNA in exercise group was lower than that in quiet group. Moderate and high intensity exercise could induce the expression of ERR 伪 mRNA in myocardium. The change of ERR 伪 mRNA in soleus muscle was different from that in myocardium, and the lowest in high intensity group. Conclusion 12 weeks exercise intervention can significantly improve cardiopulmonary endurance in rats. The effects of medium strength and high intensity sports are faster, reaching the peak at the eighth weekend and the sixth weekend, respectively. At the end of the 10th week, the small intensity group increased obviously, and reached the peak value at the 12th weekend. The exercise intervention for 12 weeks could reduce the increase of the rats' body weight. The molecular mechanism of improving cardiopulmonary endurance by exercise was mainly by increasing the expression of PGC-1 伪 in skeletal muscle rather than myocardium. The activation of AMPK and SIRT1 in myocardium and skeletal muscle was significantly affected by moderate intensity exercise and high intensity exercise, especially the expression of mRNA in AMPKG SIRT1 + PGC-1 伪 could not reflect the expression level of their respective proteins, and posttranscriptional regulation might play an important role.
【学位授予单位】：北京体育大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：G804.7

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