牛磺酸对糖尿病神经病变的保护作用

发布时间：2021-12-24 14:10

　　糖尿病已成为一个全球性的严重公共卫生问题,在中国糖尿病流行形势尤为严峻。糖尿病周围神经病变（Diabetic Peripheral Neuropathy,DPN）作为糖尿病常见的并发症之一,是一种具有对称性、长度依赖性的感觉运动性多发性神经病变。研究表明,50%到90%的糖尿病患者患有周围神经病变。因此,防治DPN已然成为了全世界重点钻研和攻克的课题之一。目前尚没有特效药物能够逆转周围神经病变的进展。牛磺酸是一种天然氨基酸,根据现有报道,它可有效对抗糖尿病引起的并发症。因此,我们选择它来治疗糖尿病神经病变,并探讨牛磺酸对糖尿病大鼠神经病变的保护作用机制。本实验分为三个部分:首先,研究牛磺酸对糖尿病大鼠神经病变的改善作用;其次,研究牛磺酸对糖尿病大鼠神经组织细胞凋亡的拮抗作用;最后,探讨牛磺酸对糖尿病大鼠神经组织miRNA表达谱的影响。将60只SD大鼠分成2组:对照组和模型组。模型组给予STZ腹腔注射建模。建模成功后将模型组分为糖尿病（DM）组、0.5%牛磺酸（T1）组、1%牛磺酸（T2）组和2%牛磺酸（T3）组。对照组与DM组给予正常饮食饮水,牛磺酸处理组分别在饮用水中加入0.5%、1... 

【文章来源】：大连医科大学辽宁省

【文章页数】：113 页

【学位级别】：博士

【文章目录】：
Abstract
Abstract-Chinese
CHAPTER 1 Taurine Ameliorated Abnormalities and Nerve Dysfunction in Diabetic Rats
    1.Background
    2.Materials and Methods
        2.1. Ethics Statement
        2.2. Establishment of animal model
        2.3. Weight gain
        2.4. Feed Intake and Water Consumption
        2.5. Latency and Motor Conduction Velocity
        2.6. Blood Glucose
        2.7. Serum Insulin
        2.8. Insulin Resistance
        2.9. Biochemical Analysis of Blood Serum
        2.10. Electron Microscopy
    3.Results
        3.1. Taurine Improved Weight Gain in STZ Induced Diabetic Rats
        3.2. Taurine lowered blood glucose in STZ induced diabetic rats
        3.3. Taurine increased HDL-C and decreased LDL-C in STZ induced diabetic rats
        3.4. Taurine Decreased Triglycerides/Triacylglycerides in STZ-induced Diabetic rats
        3.5. Serum Electrophoresis
        3.6. Serum Insulin
        3.7. Insulin Resistance
        3.8. Taurine increased MCV and reduced latency
        3.9. Taurine improved morphology of spinal cord tissue and sciatic nerve tissue
    4.Discussion
    5.Conclusion
    6.References
CHAPTER 2 Protective Effect of Taurine against Diabetes-induced Apoptosis in Spinal Cord of Rats
    1.Introduction
    2.Material and Methods
        2.1. Chemicals
        2.2. Ethics Statement
        2.3. Establishment of Animal Model
        2.4. Detection of Apoptosis
        2.5. Assay for Cytochrome c (cyt-c)
        2.6. Determination of Cyt-c Protein Expression
        2.7. Western blotting
        2.8. Determination of Caspase-3 activity
        2.9. Statistical analysis
    3.Results
        3.1. Taurine Prevented Cell Death in Diabetic Rats
        3.2. Taurine Activated Akt and Phosphorylated Bad
        3.3. Bax and Bcl-2 Expression
        3.4. Taurine Reduced the Release of Cytochrome c
        3.5. Taurine Reduced Activation of Caspase-3
    4.Discussion
    5.Conclusion
    6.References
CHAPTER 3 Effect of Taurine on mi RNA profile of Diabetic Rats
    1.Introduction
    2.Materials and Methods
        2.1. Statement of Ethics
        2.2. Animal Model
        2.3. RNA Extraction and Sequencing
        2.4. Searching for mi RNAs Significant Profiles
        2.5. Predicating the Functions of mi RNA Targets
    3.Results
        3.1. Construction of Small RNA Libraries by Sequencing
        3.2. Determination of mi RNAs Expression in Diabetic rats with or without TaurineTreatment
        3.3. Category Analysis of Specific mi RNAs in Taurine Treated Diabetic Rats
        3.4. Annotating the Function of Specific mi RNAs and Targets in Taurine Treated DiabeticRats
    4.Discussion
    5.Conclusion
    6.References
CHAPTER 4 General Background and review of literature
    1.Diabetes
    2.Taurine
        2.1. Effect of Taurine on Glucagon Activity
        2.2. Taurine Modifies Glucose Level in Diabetics
        2.3. Taurine Improves Hyperglycemia
        2.4. Taurine Improves Insulin Resistance and Secretion
    3.Diabetes Induced Complications
        3.1. Taurine Protects Brain Damage in Diabetes
            3.1.1. Taurine Protects Diabetic Neuropathy
            3.1.2. Taurine Protects Diabetic Retinopathy
        3.2. Diabetes Damages Excretory System
            3.2.1. Taurine Protects Diabetic Nephropathy
        3.3. Diabetes Damages Digestive System Organs
            3.3.1. Taurine Protects Diabetic Liver Damage
        3.4. Diabetes Damages Circulatory System
            3.4.1. Taurine Protects Diabetic Vascular Problems
            3.4.2. Taurine Protects Diabetic Heart Problems
    4.Taurine Protects Against Oxidative Stress
    5.Oxidative Stress, Taurine Mechanism
        5.1. Advanced Glycation Endproducts
        5.2. PI3Kinase/AKT pathway
        5.3. Mitochondrial Apoptosis Pathway
    6.References
ABBREVIATIONS
ACKNOWLEDGEMENT
List of Papers


【参考文献】：
期刊论文
[1]Taurine protects against retinal and optic nerve damage induced by endothelin-1 in rats via antioxidant effects[J]. Natasha Najwa Nor Arfuzir,Renu Agarwal,Igor Iezhitsa,Puneet Agarwal,Sabrilhakim Sidek,Nafeeza Mohd Ismail.  Neural Regeneration Research. 2018(11)



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