沙葱水溶性提取物对肉羊脂肪代谢相关基因表达量及甲基化的影响

发布时间：2018-01-07 11:11

本文关键词：沙葱水溶性提取物对肉羊脂肪代谢相关基因表达量及甲基化的影响　出处：《内蒙古农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：课题组前期的研究中发现,沙葱及其提取物可以降低肉羊机体内脂肪酸合成酶的活性,提高激素敏感脂酶的活性,从而影响舍饲肉羊胴体脂肪的分布和脂肪中脂肪酸的组成,并显著改善羊肉风味和品质。通过在肉羊饲料中添加沙葱水溶性提取物,研究沙葱水提物对脂蛋白脂酶(Lipoprotein Lipase,LPL),过氧化物酶体增殖体激活受体(Peroxisome proliferator-activated receptors,PPAγ)基因mRNA表达和启动子甲基化程度的影响。试验选取24只羊随机分为2组[对照组(基础日粮组)和试验组(基础日粮中添加0.1%沙葱水溶性提取物)],每组12只羊体重相近。预饲期15d,正饲期60d。正式试验结束后,每组随机选取6只样本屠宰,立即采集肉羊背部脂肪组织,进行Q-PCR测定LPL,PPARγ基因mRNA表达量。经RNA电泳图,扩增曲线及溶解曲线验证,样品结果符合试验要求,LPL和PPARγmRNA表达较对照组均有极显著差异(P0.001),LPL较对照组降低35%,相反PPAR γ较对照组增加35%。经筛选LPL基因中有4个高甲基化CpG岛记为LPLM1,LPLM2,LPLM3,LPLM4。PPARγ有一个高甲基化CpG岛。通过测序错误率分布验证以及原始数据碱基组成分布验证后分析LPL和PPARγ基因甲基化比例发现,在LPLM2中161dp试验组甲基化比例和LPLM3中32dp、57dp试验组甲基化比例显著高于对照组,分别上升32.0%,62.3%,51.9%,其LPL各位点的平均甲基化比例显著提高了 21.4%,但PPARγ甲基化比例试验组与对照组之间并无显著差异。
[Abstract]:In our previous study, it was found that Allium mongolicum and its extract could reduce the activity of fatty acid synthase and increase the activity of hormone sensitive lipase in meat sheep. Thus, the distribution of carcass fat and fatty acid composition in meat sheep were affected, and the flavor and quality of mutton were improved significantly. To study the effect of water extract from Allium sativa on lipoprotein Lipase (LPL). Peroxisome proliferator-activated receptors. The effects of PPA 纬) gene mRNA expression and promoter methylation were studied. 24 sheep were randomly divided into 2 groups. [The control group (basal diet group) and experimental group (basic diet supplemented with 0.1% water soluble extract of Allium balsica)], each group of 12 sheep body weight, 15 days prefeeding period, 60 days, after the formal trial. Six samples were slaughtered randomly in each group. The fat tissue of back of sheep was collected immediately and the expression of LPLPPAR- 纬 gene mRNA was determined by Q-PCR. RNA electrophoresis was used to detect the expression of LPLPPAR- 纬 gene. The results of amplification curve and dissolution curve showed that the expression of LPL and PPAR 纬 mRNA was significantly different from that of the control group (P0.001). Compared with the control group, LPL decreased 35%, whereas PPAR 纬 increased 35%. Four hypermethylated CpG islands in the screened LPL gene were recorded as LPLM1 / LPLM2. LPLM3. LPLM4.PPAR 纬 has a highly methylated CpG island. LPL and PPAR 纬 gene methylation ratios were analyzed by sequencing error rate distribution and base composition distribution of the original data. The methylation rate of the 161dp test group in LPLM2 and the methylation rate of the 32dp57-dp test group in LPLM3 was significantly higher than that of the control group, with a rise of 32.0% and 62.3%, respectively. The average methylation ratio of the LPL sites increased by 21.4g, but there was no significant difference between the PPAR 纬 methylation ratio test group and the control group.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S826.5

【相似文献】