固态发酵豆粕制备氨基酸复合肥的工艺研究

发布时间：2018-01-09 03:10

  本文关键词：固态发酵豆粕制备氨基酸复合肥的工艺研究　出处：《中国海洋大学》2015年硕士论文　论文类型：学位论文

  更多相关文章： 豆粕 固态发酵 氨基酸螯合中微量元素 叶面肥 大田试验

【摘要】：氨基酸复合肥是以大量元素原料与以氨基酸为配体与中微量元素金属离子络合形成的螯合物原料复配得到的一种全元素复合肥料。氨基酸中微量元素螯合物化学及生化稳定性高,拥有很好的配伍性。本文以豆粕为蛋白原料,利用实验室保存的产蛋白酶菌种WY-3,通过固态发酵的方式对豆粕进行降解,水提后得到氨基酸溶液。在适宜条件下将氨基酸与中微量元素金属离子进行水系复合螯合,得到氨基酸中微量元素螯合物。按照不同作物的营养需求进行科学复配,制备出全元素的氨基酸螯合叶面肥,并进行大田试验,检验肥效。本文通过单因素实验、响应面优化等统计学分析方法,先对固态发酵降解豆粕蛋白质的可行性及最优条件进行了分析研究,实验结果显示实验室保存的产蛋白酶菌种WY-3可对豆粕进行良好的降解。通过优化分析,结果证明在pH为6.5-7.0,料液比为1：0.7-1：0.8,温度为32℃,接种量为5%的条件下,发酵9-16天豆粕蛋白降解度达到最大,蛋白溶出率高达95%以上。通过对固态发酵工艺进一步放大设计,找到了一条成本低廉、成熟稳定的工艺路线。以25L塑料圆桶发酵方式为例,通过实验发现料层厚度、接种量、料液比是对发酵结果影响最为重要的三个因素。通过优化发现,当接种量为10%,料层厚度为15cm,水：豆粕=0.8：1,pH保持自然状态下时,在室温下发酵6天的条件可实现发酵产物蛋白溶出率80%以上的效果。在发酵完成后,采用三足刮刀式离心机离心、旋蒸浓缩等工艺可将水溶物进行大规模提取和浓缩,得到螯合所用的配体氨基酸原料。以肥料当中常用的中微量元素金属盐为原料,通过三步螫合法,即复合螯合、单独螯合、最后复配等过程很好的解决了各元素之间的配伍性问题。通过对不同作物的营养需求进行研究、结合实际种植试验制定出合适的全元素配方,按照配方比例进行复配,制备出一种全水溶性的氨基酸螯合中微量元素复合叶面肥。通过大田试验检验肥效,其增产效果明显,并利用发酵废弃物制备得到一种有机型冲施复合肥。
[Abstract]:Amino acid compound fertilizer is a kind of all-element compound fertilizer which is formed by chelate material complexing with amino acid as ligand and middle trace element metal ion. Chemistry of trace element chelate in amino acid. And high biochemical stability. In this paper, soybean meal was used as protein raw material, and the protease producing strain WY-3 was used to degrade soybean meal by solid-state fermentation. After water extraction, amino acid solution was obtained. Under suitable conditions, amino acids were chelated with metal ions in water system. The chelate of trace elements in amino acids was obtained. According to the nutritional requirements of different crops, the amino acid chelate foliar fertilizer of all elements was prepared, and the field experiment was carried out. Through single factor experiment, response surface optimization and other statistical analysis methods, the feasibility and optimal conditions of solid-state fermentation for protein degradation of soybean meal were studied. The results showed that the protease producing strain WY-3 could degrade soybean meal well. The results showed that the pH was 6.5-7.0. When the ratio of material to liquid was 1: 0.7-1: 0.8, the temperature was 32 鈩，

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