东北杏种质资源多样性研究

发布时间：2018-01-09 09:08

  本文关键词：东北杏种质资源多样性研究　出处：《沈阳农业大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： 东北杏 种质资源 表型性状 SSR标记 多样性

【摘要】：东北杏是集观赏、经济、用材于一体的重要树种,长期以来一直处于野生和半野生状态,鲜有相关研究报道。本文对东北杏主要分布区内种质资源状况开展了调查,对典型样木进行了表型性状观测和SSR分子标记分析,在此基础上分析了种质资源的形态多样性和遗传多样性。主要研究结果如下:(1)26个东北杏数量性状指标的变异系数在9.18%～66.18%之间,变异较大,表现出较高的遗传多样性。依据26个数量性状和9个质量性状指标对供试东北杏进行聚类分析,44种质分为5大类,结果体现了种源与形态特征的综合作用,种源在分类中表现出比较明显的规律。(2)采用L16(45)正交实验设计,研究了既适合东北杏也适合西伯利亚杏、野杏及普通杏的SSR-PCR反应体系。优化反应体系为:总反应体系为20μL,其中DNA模板 20ng,Mg2+2.5mmol·L-1,dNTPs0.3mmol·L-1,引物 0.15μmol L-1,Taq酶 1U。并筛选141对引物进行了全基因组SSR分子标记的开发。(3)141对引物对东北杏群体进行SSR分子标记,条带清晰度高、多态性好的引物共115对引物,SSR标记上表现出较高水平的多态性,115个标记平均等位位点数目(NA)为9.167,分布范围为2～24,有效等位位点数目(NE)为4.979,I=1.641,多态信息含量(PIC)平均值为0.689。平均期望杂合度(HE)及发现杂合度(Ho)分别为0.804和0.270。应用不加权成对算术平均法(UPGMA)将67东北杏种质聚为4类,67个东北杏样本需要12个引物才能完全分开。基于贝叶斯数学模型所确定的亚群体结构分析表明,67个东北杏种质构成的群体被分为4个亚群。且与基于SSR分子标记的聚类结果以及地理种源显著相关。上述研究结果为东北杏种质资源保护、开发利用和遗传育种工作提供了一定的科学依据,也为进一步研究提供了参考。
[Abstract]:Northeast apricot is ornamental, economic, in one of the important timber tree species, has long been a wild and semi wild state, related research reports rarely. This paper is mainly distributed in Northeast Apricot Germplasm resources situation in the region to carry out the investigation, the typical sample trees were analyzed observation and SSR molecular markers based on phenotypic traits. Analysis of the morphological diversity of germplasm resources and genetic diversity. The main results are as follows: (1) the coefficient of variation of quantitative traits of 26 large northeast apricot variation in 9.18% ~ 66.18% between, showed high genetic diversity. On the basis of 26 quantitative traits and 9 quality traits by cluster analysis for try the Northeast apricot, 44 were divided into 5 categories, the results reflected the combined effects of provenance and morphological characteristics, provenance showed obvious regularity in the classification. (2) by L16 (45) orthogonal experimental design, the research is suitable He is also suitable for Siberia northeast apricot apricot, wild apricot and SSR-PCR reaction system of apricot. Optimization of the reaction system: the total reaction system is 20 L, the DNA Mg2+2.5mmol template 20ng, L-1, dNTPs0.3mmol, L-1, 0.15 mol primers L-1, Taq enzyme 1U. and 141 pairs of primers were screened for the development of whole genome SSR molecular markers (3). 141 pairs of northeast apricot groups SSR molecular marker bands, high resolution, good a total of 115 polymorphic primers, SSR markers showed a high level of polymorphism, 115 average marker allele number (NA) was 9.167, the distribution range is 2 ~ 24, the effective number of alleles (NE) was 4.979, I=1.641, polymorphism information content (PIC) with an average of 0.689. the average expected heterozygosity (HE) and heterozygosity (Ho) was 0.804 and 0.270. respectively using unweighted pair arithmetic average (UPGMA) 67 northeast Apricot Germplasm were clustered into 4 groups. 67 Northeast Apricot samples need 12 primers to completely separate. Analysis shows that the subgroup structure of Bayesian model based on the determined 67 northeast Apricot Germplasm groups were divided into 4 subgroups. And significantly correlated with the clustering results based on SSR markers and geographical provenances. The results of the study for the protection of the Northeast Apricot Germplasm Resources and provide a scientific basis for the development and utilization of genetic and breeding work, but also provides a reference for further research.

【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S662.2
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本文编号：1400791