甲基乙二醛在围产期奶牛酮病群体监测中的作用

发布时间：2018-01-14 13:22

本文关键词：甲基乙二醛在围产期奶牛酮病群体监测中的作用　出处：《西北农林科技大学》2017年硕士论文　论文类型：学位论文

【摘要】：围产期奶牛酮病作为重要的多发性和群发性疾病,为乳业造成了严重的经济损失。因此,对围产期奶牛酮病进行群体监测和早期预警显得尤为重要。甲基乙二醛(methylglyoxal,MGO)作为一个高活性α-氧代醛,在糖尿病及其并发症中起着重要作用,被认为是疾病发展的一个生物标志物。酮病奶牛血液中是否能够产生MGO,其在代谢中发挥什么作用?目前仍不清楚。为明确血清MGO是否与奶牛酮病有相关性,本论文通过检测奶牛血清中糖、脂质、矿物元素代谢等相关指标,以及日粮营养水平和生产性能等指标,筛选和确定围产期奶牛酮病群体监测的预警体系,探讨血清MGO在奶牛酮病群体监测中的作用。结果如下:从产前2周开始,围产期奶牛血清中BHBA、NEFA和AST浓度逐渐升高,产后1周(第7 d)达到最高,此后逐渐降低;血清中GLU、Ca、K和P浓度从产前2周开始逐渐降低,产后1周最低,此后逐渐恢复;围产期奶牛,产前BCS和血清中GLU、Mg和K与产后水平呈显著正相关;泌乳早期(产后第14 d),围产期奶牛血清中NEFA与牛如中MF、F/P呈显著正相关,与MP呈显著负相关;GLU与日粮中DM、EE、CF、NDF、ADF和MUN呈显著负相关,与GE呈显著正相关;BCS与MF、ML和TS呈显著正相关,与日粮中DM、EE、CF、NDF和ADF呈显著负相关;干奶天数与产后1周血清中BHBA和NEFA浓度呈显著正相关。泌乳早期,与健康奶牛相比,酮病奶牛干奶天数,血清中BHBA、NEFA,牛乳中F/P显著增加,MP显著降低。酮病奶牛血清中MGO浓度显著高于青年奶牛和健康奶牛;与青年奶牛相比,酮病奶牛血清中GLU、FA、TG、K、P和Mg浓度均显著降低,DHAP、NEFA、BHBA、AST、TBIL和Hp含量显著升高;试验奶牛血清中NEFA与GLU、FA、K、P和Mg浓度呈显著负相关,与AST、TBIL、Hp和DHAP显著正相关;BHBA与GLU、FA、TG、Mg和K呈显著负相关,与AST、TBIL、Hp和DHAP显著正相关;MGO与GLU、FA、TG、K、P和Mg呈显著负相关,与BHBA、NEFA、AST、TBIL、Hp和DHAP呈显著正相关;泌乳奶牛血清中MGO与GLU和FA呈显著负相关,与BHBA、NEFA、TG、Hp和P呈显著正相关。结论:泌乳早期,血清中BHBA和NEFA可作为监测酮病的首选指标,血清中GLU、AST,BCS,MF、MP和F/P可作为监测酮病的辅助指标。酮病奶牛血清中MGO产生的增加,可能来源为脂质代谢产生的酮体和甘油糖异生生成的磷酸二羟丙酮;MGO可作为奶牛酮病群体监测中的一个生物标志物。
[Abstract]:Perinatal ketosis in dairy cows, as an important multiple and mass disease, has caused serious economic losses for the dairy industry. Group monitoring and early warning of perinatal ketosis in dairy cattle is particularly important. Methyl Glyoxal methylglyoxalMGOA is a highly active 伪 -oxaldehyde. It plays an important role in diabetes mellitus and its complications and is considered to be a biomarker of disease development. Can MGOs be produced in the blood of ketosis cows and what role does MGOs play in metabolism? It is still unclear. In order to determine whether serum MGO is related to ketosis in dairy cows, this paper detected the serum glucose, lipid, mineral elements metabolism and other related indicators. As well as dietary nutrition level and production performance indicators to screen and determine the perinatal cow ketodisease monitoring early warning system. To explore the role of serum MGO in the monitoring of ketopathy in dairy cattle. The results are as follows: from the second week before delivery, the serum concentration of MGO and AST in the perinatal dairy cattle gradually increased. The peak was reached at 1 week postpartum (7 d), and then decreased gradually. The concentrations of GLU CaK and P in serum decreased gradually from 2 weeks before delivery to the lowest in 1 week postpartum, and then recovered gradually. In perinatal dairy cows, BCS and GLU mg and K in serum were positively correlated with postpartum levels. At the early stage of lactation (postpartum 14th day), the serum NEFA in perinatal cows was positively correlated with that in cattle such as MFF / P, and negatively correlated with MP. There was a significant negative correlation between GLU and ADF and MUN, and a significant positive correlation between GLU and GE. There was a significant positive correlation between BCS and MFML and TS, and a significant negative correlation between BCS and CFD and ADF in diet. The days of dry milk were positively correlated with the concentration of BHBA and NEFA in serum at 1 week postpartum. The concentration of MGO in serum of ketosis cows was significantly higher than that of young cows and healthy cows. Compared with the young cows, the serum concentrations of GLU / FAA, TGG, P and mg in ketosis cows were significantly lower than those in young cows. The contents of TBIL and HP increased significantly. There was a significant negative correlation between serum NEFA and GLU FAKP and mg concentration, and a positive correlation between NEFA and TBILHP and DHAP. BHBA was negatively correlated with mg and K, and positively correlated with HP and DHAP. There was a significant negative correlation between MGO and TGG P and mg, and a significant positive correlation between MGO and HP and DHAP. There was a significant negative correlation between serum MGO and GLU and FA in lactating cows, and a significant positive correlation with HP and P in NEFAA. Conclusion: early lactation. Serum BHBA and NEFA can be used as the first choice for monitoring ketosis. MP and F / P can be used as auxiliary indexes for monitoring ketosis. The increase of MGO production in serum of ketosis cows may come from the ketone body produced by lipid metabolism and dihydroxyacetone phosphate produced by glycerol glycometabolism. MGO can be used as a biomarker for monitoring of ketosis in dairy cattle.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.23

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