不同抗生素替代物对保育猪生产性能、免疫和盲肠微生物的影响

发布时间：2018-02-11 05:11

本文关键词： 保育猪抗生素替代物生产性能免疫性能盲肠微生物　出处：《山东农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：本试验旨在研究不同抗生素替代物及其部分组合对保育猪生产性能、免疫及肠道健康的影响及作用机理,为微生态制剂、三丁酸甘油酯、抗菌肽和葡萄糖氧化酶及其部分组合在生猪生产上的应用提供数据,也为实现生猪的无抗养殖提供理论依据。试验采用单因子试验设计,选取40日龄“杜×长×大”断奶仔猪320头,随机分为8个处理,每个处理4个重复,每个重复10头,对照组饲喂基础饲粮,试验组在基础饲粮中分别添加生产厂家推荐添加量的抗生素、三丁酸甘油酯、微生态制剂、抗菌肽和葡萄糖氧化酶以及微生态制剂+抗菌肽、微生态制剂+葡萄糖氧化酶的组合,试验预试期7 d,正式期120d。结果表明:由各处理组的腹泻率与死淘率、平均日增重、料重比的表现以及肠道重量指数的表现得知,抗生素、微生态制剂、三丁酸甘油酯、抗菌肽和葡萄糖氧化酶以及微生态制剂+葡萄糖氧化酶、微生态制剂+抗菌肽均能改善保育猪生产性能(P0.05),而与抗生素相比较,微生态制剂、三丁酸甘油酯、葡萄糖氧化酶、微生态制剂+葡萄糖氧化酶、微生态制剂+抗菌肽在生产性能上具有明显优势,而抗菌肽与抗生素相比优势不明显;由免疫球蛋白IgG含量和脾脏指数反映出,微生态制剂+葡萄糖氧化酶、微生态制剂+抗菌肽、三丁酸甘油酯、抗菌肽日粮均能在这两方面增强保育猪的免疫力,而使用葡萄糖氧化酶、微生态制剂与使用抗生素相比在这两方面没有显著差异(P0.1);并且通过高通量法测序得出结果,葡萄糖氧化酶、微生态制剂+葡萄糖氧化酶、抗生素和微生态制剂可以利用提高Prevotella(普雷沃菌属)的比例来使日粮营养元素更加丰富。综上所述,在本试验条件下不添加抗生素,单独添加微生态制剂、三丁酸甘油酯、抗菌肽和葡萄糖氧化酶以及微生态制剂+葡萄糖氧化酶、微生态制剂+抗菌肽均能将保育猪成功养殖,并且微生态制剂、三丁酸甘油酯、葡萄糖氧化酶、微生态制剂+葡萄糖氧化酶、微生态制剂+抗菌肽比抗生素效果更好。
[Abstract]:The purpose of this study was to study the effects and mechanism of different antibiotic substitutes and their combinations on the production performance, immunity and intestinal health of conservation pigs. The application of antimicrobial peptide, glucose oxidase and some combinations in the production of live pigs provides data, and also provides a theoretical basis for the realization of non-resistant breeding of live pigs. 320 weaned piglets of 40 days old were randomly divided into 8 treatments, 4 replicates in each treatment and 10 in each. The control group was fed with basic diet. The experimental group added antibiotics, glycerol tributyrate, microecological agents, antimicrobial peptides and glucose oxidase and antimicrobial peptides recommended by manufacturers to the basic diet. The combination of glucose oxidase, a microecological preparation, was tested at 7 days and 120 days. The results showed that the diarrhea rate, the death rate, the average daily gain, the performance of feed weight ratio and the intestinal weight index of each treatment group were determined. Microecological preparations, tributyric glycerides, antimicrobial peptides and glucose oxidase, and microecological agents, glucose oxidase and microecological antimicrobial peptides can all improve the production performance of conservation pigs (P 0.05), but compared with antibiotics, microecological preparations, Triglyceride, glucose oxidase, glucose oxidase, microecological agent glucose oxidase, microecological preparation antimicrobial peptide have obvious advantage in production performance, but antibacterial peptide has no obvious advantage compared with antibiotic. The content of immunoglobulin IgG and spleen index showed that glucose oxidase, antimicrobial peptide, tributyrate and antimicrobial peptide diet could enhance the immunity of protecting pigs in these two aspects. However, there was no significant difference between microecological agents and antibiotics in the use of glucose oxidase, and the results of high throughput sequencing showed that glucose oxidase, microecological agent glucose oxidase, glucose oxidase, Antibiotics and microecological agents can be used to increase the proportion of Prevotella (Prevotella) to make dietary nutrients more abundant. Antimicrobial peptides and glucose oxidase, as well as microecological agents glucose oxidase, microecological agents antimicrobial peptides can be successfully cultured conservation pigs, and microecological agents, tributyric glycerol ester, glucose oxidase, Glucose oxidase and antimicrobial peptide were more effective than antibiotics.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S828.5

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