水稻籽粒锌富集主要农艺调控因子研究

发布时间：2018-02-13 20:01

本文关键词： 锌生物有效性叶面锌肥内生菌土壤类型品种差异　出处：《浙江大学》2017年硕士论文　论文类型：学位论文

【摘要】：本研究通过大田试验,筛选与鉴定适宜在浙江省栽种的水稻富锌品种,研究纳米-有机锌叶面肥喷施对水稻籽粒中锌积累的影响,并通过人体细胞系体外试验技术探明喷肥对水稻籽粒中锌的生物有效性的影响。室内盆栽试验研究接种特异内生菌与土壤类型差异对水稻籽粒中锌积累与生物有效性的交互作用与影响,主要结果总结如下:1.通过对30个水稻品种的大田试验,筛选出锌富集、低植酸及高蛋白质含量的优质水稻品种1个,钱优0506。2.大田喷施纳米-有机锌叶面肥对水稻籽粒锌富集影响试验结果表明,纳米-有机锌叶面肥不同程度地提高了各水稻品种的穗重和生物产量;提高精米中锌富集浓度,降低了精米中植酸含量、提升了精米中蛋白质含量。喷肥后不同水稻品种精米锌含量为15.56～30.65 mg ·kg-1,其中秀水134、深两优884、浙粳88的增幅均在30%以上。人体细胞系体外试验结果表明喷肥后不同水稻品种精米中锌被人体细胞吸收率增幅为14.12%～22.91%,生物可利用锌总量增幅为14.88%～41.34%。3.接种特异内生菌促进水稻苗期植株的生长发育,且增强植株对锌的吸收,其中苗期植株地上部锌的增幅为2.31%~42.21%,地下部锌的吸收量增幅为3.76%～26.09%。4.锌超积累植物分离得到的内生菌对水稻籽粒锌及重金属吸收积累影响明显。在不同土壤上栽培时,接菌处理后水稻精米中的锌含量、蛋白质、铁含量以及钙含量均有一定程度的增加,同时,接菌处理可以降低精米中植酸的含量。此外,人体细胞系体外试验表明,所选试验组合中精米锌的吸收率范围为7.65%～12.45%,生物可利用锌总量范围为1.63～4.10 μg·g-1。未接菌时,紫色土上栽培的浙优12号(锌高富集品种)与浙优18(锌低富集品种)的细胞生物有效性最佳;接菌后,红壤上栽培的浙优12号精米锌吸收率增加了 33.44%,生物可利用锌总量增加了 64%,而浙优18精米锌吸收率增加了 25.62%,生物可利用锌总量增加了 75.46%。表明水稻籽粒锌富集较大程度上受内生菌与土壤性质的交互作用影响。
[Abstract]:In this study, the zinc rich rice varieties suitable for planting in Zhejiang Province were screened and identified through field experiments. The effects of nano-organic zinc foliar fertilizer spraying on zinc accumulation in rice grains were studied. The effects of spraying fertilizer on the bioavailability of zinc in rice grain were investigated by using human cell line test in vitro. Effects of inoculation of specific endophytic bacteria and soil type on zinc accumulation and bioavailability in rice grain were studied in pot experiment in laboratory. The interaction and influence of effectiveness, The main results were summarized as follows: 1 high quality rice variety with zinc enrichment, low phytic acid and high protein content was selected by field experiment of 30 rice varieties. Effects of Nano-Organic Zinc foliar Fertilizer on Grain Zinc enrichment in Rice (Oryza sativa L.). The results showed that Nano-Organic Zinc foliar Fertilizer increased the ear weight and biological yield of rice varieties in varying degrees, and increased Zn concentration in milled rice. The content of phytic acid in milled rice was reduced, The zinc content of different rice varieties after spraying fertilizer was 15.56 ~ 30.65 mg 路kg ~ (-1), in which Xiushui 134, Shenliangyou 884 and Zhejiang Japonica 88 increased more than 30%. The results of human cell line test in vitro showed that different water content was different after spraying fertilizer. The absorption rate of zinc in rice milled rice was increased by 14.12% and 22.91%, and the total amount of zinc was increased by 14.88% ~ 41.34% 路3.The inoculation of specific endophytic bacteria promoted the growth and development of rice plants at seedling stage. And to enhance the absorption of zinc by plants, In the seedling stage, the increase of Zn in shoot part was 2.31% and that in underground part was 3.76 ~ 26.09.4.The effect of endophytic bacteria isolated from the plant on the accumulation of Zn and heavy metal in rice grain was obvious. When cultivated on different soil, the growth rate of Zn uptake and heavy metal accumulation in rice grain was significant. The content of zinc, protein, iron and calcium in rice milled rice increased to some extent after inoculation, and the phytic acid content in milled rice was decreased by inoculation. The absorptivity of zinc in milled rice was 7.65 ~ 12.45 and the total amount of zinc was 1.63 ~ 4.10 渭 g 路g ~ (-1). Without inoculation, the cell bioavailability of Zhe you 12 (high zinc enriched variety) and Zhejiang you 18 (low zinc enriched variety) cultivated on purple soil was the best. After inoculation, The zinc absorption rate of Zhe you 12 milled rice cultivated on red soil increased 33.4444%, the total amount of bioavailable zinc increased 64%, the Zn-uptake rate of Zhe you 18 milled rice increased 25.62% and the total amount of bioavailable Zn increased 75.46%. The collection was largely affected by the interaction between endophytic bacteria and soil properties.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S511

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