白檀组织培养技术研究

发布时间：2018-02-27 10:12

  本文关键词： 白檀 组织培养技术 茎段 胚　出处：《吉林农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：白檀(Symplocos paniculata)为山矾科(Symplocaceae)山矾属落叶灌木或小乔木,花朵洁白繁多,亮蓝果实密布树冠,树形优美,具有较高的园林观赏价值。同时,全株在工、医、农业等方面均有广泛用途,是集观赏价值和经济价值于一身的优良野生资源。为了能够更好地开发、保护和综合利用白檀野生资源,高效、稳定的白檀组织培养技术体系亟待建立。本文以白檀不同部位的带有腋芽的茎段和种子为外植体,对外植体消毒、启动培养、增殖培养、生根培养、炼苗移栽的影响因素进行研究,建立了白檀组织培养体系。主要研究结果如下:1.带腋芽嫩茎为最适宜的外植体,最佳灭菌方案为0.1%升汞溶液灭菌3分钟,污染率可控制在18.2%;白檀胚做外植体,最佳预处理为浸泡在1100mg/L GA3溶液中24h,经75%酒精浸泡30s,再用0.1%升汞溶液灭菌7min,可将污染率控制在9.2%。2.嫩茎的最佳启动培养基为1/2MS+0.5mg/L 6-BA+0.1mg/L NAA,启动率达87.44%;白檀胚经预处理及灭菌处理后,接种到1/2WPM培养基,启动率达78.59%。3.采用丛生芽增殖途径对白檀试管苗进行继代增殖培养,试管苗最适增殖培养基为WPM+1.7mg/L ZT,平均增殖系数可达5.03,芽平均高度达5.00cm。4.双芽茎段最适做白檀试管苗增殖材料;25℃/15℃(昼12h/夜12h)温差处理可使白檀试管苗生长健壮。5.白檀试管苗生根最适培养基为WPM+0.5mg/L IBA+0.5mg/L NAA+20g/L蔗糖+2g/L活性炭+8g/L琼脂,生根率达93%,平均生根数量为6根。6.白檀试管苗最适移栽基质为体积比1:1的园土和草炭土混合基质,移栽成活率为83%。
[Abstract]:Symplocos paniculata) is a deciduous shrub or small tree of the genus Symplocaceae. It has a wide range of white flowers, bright blue fruit, dense crown, beautiful tree shape and high ornamental value in gardens. At the same time, the whole plant is widely used in engineering, medicine, agriculture and so on. It is an excellent wild resource with both ornamental value and economic value. In order to develop, protect and make comprehensive use of the wild resources of white sandalwood, it is highly effective. It is urgent to establish a stable tissue culture system. In this paper, the stem segments and seeds with axillary buds were used as explants to sterilize the explants, start culture, proliferate culture, and root culture. The main results are as follows: 1. The young stem with axillary bud is the most suitable explant, and the best sterilization scheme is 0.1% mercuric solution for 3 minutes. The pollution rate can be controlled at 18.2cm, and the embryo is used as explant. The best pretreatment was immersion in 1100mg / L GA3 solution for 24h, soaking in 75% alcohol for 30s, sterilizing with 0.1% mercuric solution for 7 mins. The pollution rate was controlled at 9.2 mg / L. The best starting medium for tender stem was 1 / 2ms 0.5 mg / L 6-BA 0.1 mg / L NAA, the priming rate was 87.444.After pretreatment and sterilization, the best starting medium for the tender stem was 87.444.After pretreatment and sterilization, the best starting medium for the tender stem was: 1 / 2 MS, 0.5 mg / L 6-BA 0.1 mg / L NAA. When inoculated to 1 / 2 WPM medium, the initiation rate was 78.59.3.The propagation pathway of cluster buds was used to subculture the test-tube plantlets of sandalwood. The optimum culture medium was WPM 1.7 mg / L ZT, the average multiplication coefficient was 5.03, and the average height of buds was 5.00 cm. 4. The best medium for the growth of white sandalwood seedlings was 25 鈩，

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