平菇优良菌株的选育及评价

发布时间：2018-03-11 04:14

本文选题：平菇　切入点：单孢分离　出处：《河北工程大学》2017年硕士论文　论文类型：学位论文

【摘要】：本试验收集了冀中南地区33个平菇栽培菌株,通过酯酶同工酶、ISSR分子标记分析、拮抗试验以及出菇试验,对33个平菇栽培菌株进行了生理生化和分子标记鉴定。在此基础上,选取了生产性状优劣互补的“99”、“680”和“双抗”三个菌株作为亲本。通过单孢杂交配对和原生质体融合,共获得176个杂交子代菌株,并对其进行一些列筛选试验,旨在获得平菇优良菌株。1.通过拮抗测定、ISSR分子生物标记分析和酯酶同工酶酶谱为依据,将供试菌株分为7大类,其中栽培量最大的是以“89”、“99”和“双抗黑平”为代表的三大类菌株。2.通过单孢分离技术分离了三个菌株的担孢子,得到“99”单核体2个,“680”单核体5个,“双抗”单核体3个。选取菌丝生长较快的亲本“99”和“680”单核菌丝分别与“双抗”单核菌丝进行了两两杂交配对,得到21个杂交组合菌株。3.根据锁状联合有无进一步确定杂交子,筛选出了10个优良杂交子,采用拮抗、酯酶同工酶及出菇等方法对融合子进行了进一步鉴定和评价分析。10个杂交子均与亲本有明显的拮抗线,且具有两亲本菌株的典型酯酶同工酶条带,确定是杂交菌株,以此为依据进一步筛选出了4个平菇优良杂交子,都具备出菇能力,分别编号为“Pz945”,“Pz935”,“Pz675”,“Pz6115”。4.同时制备平菇优良品种“99”和“双抗”原生质体,并分离了单核体进行融合。得到融合子155个,根据锁状联合有无进一步确定融合子,通过菌丝长势筛选出14个长势较好的融合。
[Abstract]:In this experiment, 33 cultivated strains of Pleurotus ostreatus were collected in the middle and south of Hebei Province. The physiological, biochemical and molecular markers of 33 cultivated strains of Pleurotus ostreatus were identified by ISSR molecular marker analysis, antagonistic test and mushroom production test. On the basis of this, 33 cultivated strains of Pleurotus ostreatus were identified by physiological, biochemical and molecular markers. Three strains of "99", "680" and "double resistance" with complementary production traits were selected as parents. 176 hybrid progenies were obtained by single sporulation pairing and protoplast fusion, and some screening tests were carried out on them. The purpose of this study was to obtain fine strains of Pleurotus ostreatus. Based on the analysis of ISSR molecular biomarkers and esterase isozyme patterns, the tested strains were divided into 7 groups. Among them, the most cultivated strains were "89", "99" and "double resistance to black flat". Two monokaryons of "99", five monosomes of "680" and three monosomes of "double antibody" were obtained. 21 hybrid combination strains. 3. According to the combination of lock-like combination to further determine the hybrids, 10 excellent hybrids were screened, and antagonistic methods were used. The fusion was further identified and evaluated by esterase isozyme and mushroom extraction methods. The 10 hybrids had obvious antagonistic lines with their parents, and the typical esterase isozyme bands of two parent strains were identified as hybrid strains. On this basis, four fine hybrids of Pleurotus ostreatus were further selected, all of which had the ability to produce Pleurotus ostreatus, numbered "Pz945", "Pz935", "Pz675", "Pz6115" .4.At the same time, the protoplasts of "99" and "double resistance" of Pleurotus ostreatus were prepared. According to the combination of lock-like or not, 14 fusants with good growth were screened by hyphal growth.
【学位授予单位】：河北工程大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S646.14

【参考文献】