复合微生态制剂对刺参幼参生长、水质及免疫相关酶的影响

发布时间：2018-03-13 08:01

本文选题：饲料　切入点：刺参幼参　出处：《大连海洋大学》2017年硕士论文　论文类型：学位论文

【摘要】：为探究饲料中添加不同种类的复合微生态制剂对刺参Apostichopus japonicus幼参生长、水质及免疫相关酶的影响,用中草药(黄芪、大青叶、穿心莲、当归、陈皮、炒山楂、三七与金银花)与益生菌(硝化细菌Nitrobacteria、光合细菌PSB、枯草芽孢杆菌Bacillus subtilis、海洋红酵母Rhodotorula sp.)分别组合成5种不同的复合微生态制剂并与市售英伟(芽孢杆菌)以1%的饲料添加比例于常规投喂体质量为(0.5~5g)的幼参1周后定期投喂3周,记为试验组,并设只投喂常规饲料的对照组,试验期间每隔7d测定一次相关水质指标,试验4周左右后,测定幼参生长指标与免疫相关酶活性。结果表明:除D组(益生菌组)外,其余试验组存活率(SR)与K组(对照组)相比差异不显著(P㧐0.05),B、C组(中草药+益生菌组)稍高,且组间差异不显著(P㧐0.05),F组(英伟组)增重率(BWG)及特定增长率(SGR)为最高,效果较好;水质方面各组水体理化指标呈不断升高的趋势,但降解氨态氮(NH3-N)方面试验后期F组(英伟组)与B组(中草药+益生菌组)、C组(中草药+益生菌组)及E组(益生菌组)差异不显著(P0.05),降解亚硝态氮(NO2--N)方面F组(英伟组)效果较好,各组水体可溶性性活性磷(PO4-P)含量普遍增加;免疫酶方面,各试验组免疫酶活性普遍高于对照组,其中A组(中草药组)、F组(市售英伟组)碱性磷酸酶(AKP)活性显著高于K组(对照组)(P㩳0.05),且组间差异不显著(P㧐0.05),C组(中草药+益生菌组)酸性磷酸酶(ACP)活性为最高,过氧化氢酶(CAT)活性D组(益生菌组)相对较低,但D组(益生菌组)溶菌酶(LSZ)活性为最高,其余各组间差异不显著(P㧐0.05),A组(中草药组)总超氧化物歧化酶(T-SOD)活性为最高,显著高于其余各组(P㩳0.05)。试验结果表明,饲料中添加一定比例不同种类的复合微生态制剂对刺参幼参的生长及水质改良有一定的促进作用,且能够显著提高刺参幼参一些免疫相关酶的活性。
[Abstract]:In order to investigate the effects of different kinds of compound microecological agents on the growth, water quality and immune-related enzymes of Apostichopus japonicus young ginseng, Chinese herbal medicines (Astragalus membranaceus, Radix Isatidis, andrographis paniculata, Angelica sinensis, Radix angelicae, Pericarpium sinensis, fried Hawthorn) were used. Panax notoginseng and honeysuckle) and probiotics (nitrifying bacteria Nitrobacter sp., photosynthetic bacteria PSBs, Bacillus subtilis Bacillus, marine red yeast Rhodotorula sp. The feeding ratio was 3 weeks after 1 week, when the body weight of the regular feeding was 0.5g. It was recorded as the experimental group, and the control group was only fed with conventional feed. During the experiment, the relevant water quality indexes were measured every 7 days, and after 4 weeks of test, The results showed that the survival rate of group D (probiotics) was not significantly different from that of group K (control group) except for group D (probiotics). Group C (Chinese herbal probiotics group) was slightly higher, and the difference between groups was not significant (P > 0.05). The weight gain rate (BWGR) and specific growth rate (SGR) were the highest in group F (Yingwei group), and the effect was better, and the physicochemical index of water in each group showed an increasing trend in water quality. However, there was no significant difference between group F (Yingwei group) and group B (probiotics group of Chinese herbal medicine) and group E (probiotics group) in the degradation of ammonia nitrogen (NH3-N). (Yingwei group) has a better effect. The content of soluble active phosphophosphate (PO4-P4) in the water of each group increased generally, and the activity of immune enzyme in each experimental group was generally higher than that in the control group. The activity of alkaline phosphatase (AKP) in group A (Chinese herbal medicine group) and group F (marketable Yingwei group) was significantly higher than that in group K (control group). The difference between groups was not significant (P > 0.05). The activity of acid phosphatase (ACPP) was the highest in group C (probiotics group) and lower in group D (probiotics group), but the activity of lysozyme LSZ was the highest in group D (probiotics group). The activity of total superoxide dismutase (T-SOD) in group A (Chinese herbal medicine) was the highest, which was significantly higher than that in other groups. The results showed that the addition of certain proportion of different kinds of compound microecological agents to the feed could promote the growth and water quality improvement of young ginseng, and could significantly improve the activities of some immune-related enzymes of young ginseng.
【学位授予单位】：大连海洋大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S968.9

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