基于生理指标分析唐古特白刺抗旱优良家系的早期选择

发布时间：2018-03-21 09:37

本文选题：唐古特白刺　切入点：优良家系　出处：《甘肃农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：本研究采集我国甘肃酒泉、张掖、兰州、武威唐古特白刺天然分布区的种子,分别建立白刺种源试验,初选相同的31个3a生唐古特白刺家系为研究材料,测定与其抗旱性相关的多个生理指标,运用主成分分析和隶属函数法综合评价其抗旱性,进而筛选优良家系,以期为唐古特白刺抗旱育种材料的选育和推广提供参考依据。得出如下主要结果:1)POD、SOD、Chl a、Chl b、Chl(a+b)、Chl a/b、SP、SS、Pro、Fo、Fm、Fv、Fv/Fm、Fv/Fo、Y(Ⅱ)、NPQ和qP在31个唐古特白刺家系间变异显著,可作为唐古特白刺优良家系选择指标。2)两试点的唐古特白刺家系NPQ的变异系数最大,其次是POD和SOD变异系数较大,这几个指标是反应家系间生理状态差异性最敏感的生理指标,后期在抗旱品种选育中它们应受到的关注度最高。3)从单项指标选择结果得出,各试点单指标下选取的唐古特白刺家系相互交叉但不完全相同,说明采用单项指标选取优良家系存在片面性和无代表性。4)对唐古特白刺渗透调节物、抗氧化酶及叶绿素含量的分析中筛选的优良家系为金塔种源的j3-9和j3-8号家系、民勤沙井子种源的w3-15和w3-12号家系以及瓜州槽沿子种源的jc-8号家系。通过对抗旱性综合评价方法的筛选得出,采用隶属函数法和主成分分析中的任何一种方法均能对植物的抗旱性强弱进行客观的综合评价。5)对31个唐古特白刺家系的叶绿素荧光参数分析中筛选的优良家系为武威民勤沙井子种源的w3-12和w3-15、酒泉金塔种源的j3-9、酒泉瓜州槽沿子种源的jc-8、武威民勤小坝口的w2-20号家系。6)综合17个指标筛选得出的优良家系为金塔种源的j3-9和j3-8号家系、民勤沙井子种源的w3-15和w3-12号家系以及瓜州槽沿子种源的jc-8号家系,入选率为16.1%,其中表现最好的为jc-8和w3-15号家系。
[Abstract]:In this study, we collected seeds from natural distribution areas of Jiuquan, Zhangye, Lanzhou and Wuwei Tanggute in Gansu Province, and established the provenance experiment of Nitraria chinensis respectively. 31 families of the same 3 years old Tangut Spurs were selected as the research materials. Several physiological indexes related to their drought resistance were determined and their drought resistance was evaluated by principal component analysis and membership function method. In order to provide a reference for breeding and popularizing drought resistance breeding materials of Dangut, the main results are as follows: 1. The main results of this study are as follows: 1. The main results are as follows: 1. The main results are as follows: 1. The variation of NPQ and QP among the 31 families of Dangut Spurs is significant among the 31 families of Dangut Spurs, Chl / bSPN, ProFmFmSv, FvP, FvP, FvP, FvP, and QP, respectively, among the 31 families of Dangut Spurs, the variation of NPQ and QP is significant among the 31 families. The coefficient of variation of NPQ was the largest, followed by the coefficient of variation of POD and SOD, which was the most sensitive index to reflect the difference of physiological state among families. In the later stage of breeding drought-resistant varieties, they should receive the highest attention. 3) from the results of single index selection, it is concluded that the families of Tanggut Spurs selected under each pilot single index are intersected but not exactly the same. The results showed that there were one-sided and unrepresentative. 4) in the analysis of osmotic regulator, antioxidant enzyme and chlorophyll content of Tangut, the excellent families selected from Jinta provenance were J3-9 and J3-8. The W3-15 and w3-12 families of the manhole subprovenance of Minqin and the jc-8 pedigree of the sub-provenance along the Guazhou trough were obtained by screening the comprehensive evaluation method of drought resistance. The membership function method and any of the principal component analysis methods can be used to evaluate the drought resistance of plants objectively and synthetically. 5) the excellent families selected in the chlorophyll fluorescence parameter analysis of 31 Tangut Spurs families are selected. For the W3-12 and w3-15 provenances, j3-9 of Jinta provenance of Jiuquan, jc-8 of Guazhuanzhuanzi provenance of Jiuquan, and W2-20 pedigree of Wumiqin Xiaobakou, W3-12 and w3-15), the excellent families were J3-9 and J3-8 families of Jinta provenance. The selection rate of the W3-15 and w3-12 families and the jc-8 pedigree of Guazhou trough provenance was 16.1, among which the best were jc-8 and w3-15 families.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S793.9

【参考文献】